Novel assessment of hepatic iron distribution by synchrotron radiation X-ray fluorescence microscopy. 2010

Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan.

Excess iron deposition in the liver is known to be hepatotoxic and may exacerbate liver injury. However, little is known about iron distribution in the lobule because of the lack of a highly sensitive detection method. The aim of this study is to determine iron distribution in the lobule of human liver by means of synchrotron radiation X-ray fluorescence (SRXRF) microscopy. Mapping of the trace elements was done with use of SRXRF microscopy and compared with the results of staining by Berlin blue and oxidative stress markers. Iron deposits were distributed predominantly in periportal hepatocytes in the normal liver in a decreasing gradient from the periportal area to the perivenous area. This distribution was consistent with the formation of oxidative stress markers, suggesting that hepatocytes in the periportal area may be predominantly primed by iron-induced free radical damage even in normal liver. On the other hand, iron deposits in the periportal area were more intense than those in the centrilobular area in both the liver with chronic hepatitis C and the cirrhotic liver. In conclusion, elemental mapping by SRXRF microscopy was a highly sensitive method for the detection and mapping of elements such as iron and copper in liver sections.

UI MeSH Term Description Entries
D007501 Iron A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN. Iron-56,Iron 56
D008103 Liver Cirrhosis Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules. Cirrhosis, Liver,Fibrosis, Liver,Hepatic Cirrhosis,Liver Fibrosis,Cirrhosis, Hepatic
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013052 Spectrometry, X-Ray Emission The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods. Particle-Induced X-Ray Emission Spectrometry,Proton-Induced X-Ray Emission Spectrometry,Spectrometry, Particle-Induced X-Ray Emission,Spectrometry, Proton-Induced X-Ray Emission,Spectrometry, X-Ray Fluorescence,X-Ray Emission Spectrometry,X-Ray Emission Spectroscopy,X-Ray Fluorescence Spectrometry,Energy Dispersive X-Ray Fluorescence Spectrometry,Energy Dispersive X-Ray Fluorescence Spectroscopy,Energy Dispersive X-Ray Spectrometry,Energy Dispersive X-Ray Spectroscopy,Particle Induced X Ray Emission Spectrometry,Proton Induced X Ray Emission Spectrometry,Spectrometry, Particle Induced X Ray Emission,Spectrometry, Proton Induced X Ray Emission,Spectrometry, Xray Emission,Wavelength Dispersive X-Ray Fluorescence Spectrometry,Wavelength Dispersive X-Ray Fluorescence Spectroscopy,Wavelength Dispersive X-Ray Spectrometry,Wavelength Dispersive X-Ray Spectroscopy,X-Ray Fluorescence Spectroscopy,Xray Emission Spectroscopy,Emission Spectrometry, X-Ray,Emission Spectrometry, Xray,Emission Spectroscopy, X-Ray,Emission Spectroscopy, Xray,Energy Dispersive X Ray Fluorescence Spectrometry,Energy Dispersive X Ray Fluorescence Spectroscopy,Energy Dispersive X Ray Spectrometry,Energy Dispersive X Ray Spectroscopy,Fluorescence Spectrometry, X-Ray,Fluorescence Spectroscopy, X-Ray,Spectrometry, X Ray Emission,Spectrometry, X Ray Fluorescence,Spectroscopy, X-Ray Emission,Spectroscopy, X-Ray Fluorescence,Spectroscopy, Xray Emission,Wavelength Dispersive X Ray Fluorescence Spectrometry,Wavelength Dispersive X Ray Fluorescence Spectroscopy,Wavelength Dispersive X Ray Spectrometry,Wavelength Dispersive X Ray Spectroscopy,X Ray Emission Spectrometry,X Ray Emission Spectroscopy,X Ray Fluorescence Spectrometry,X Ray Fluorescence Spectroscopy,X-Ray Fluorescence Spectroscopies,Xray Emission Spectrometry
D017356 Synchrotrons Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant. Synchrotron
D019698 Hepatitis C, Chronic INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS. Chronic Hepatitis C
D022781 Hepatocytes The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules. Hepatic Cells,Cell, Hepatic,Cells, Hepatic,Hepatic Cell,Hepatocyte

Related Publications

Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
August 1998, Nature structural biology,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
August 1976, Applied optics,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
December 1999, The American journal of physiology,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
January 1988, Ultramicroscopy,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
January 2023, Methods in molecular biology (Clifton, N.J.),
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
November 2012, Journal of synchrotron radiation,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
January 2002, Analytical chemistry,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
June 2023, Scientific reports,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
August 2019, Analytical chemistry,
Hisoka Kinoshita, and Yuichi Hori, and Takumi Fukumoto, and Takuji Ohigashi, and Kunio Shinohara, and Yoshitake Hayashi, and Yonson Ku
December 2003, European radiology,
Copied contents to your clipboard!