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The DNA untwisting enzyme has been partially purified from Saccharomyces cerevisiae. The enzyme exhibits a pH optimum of 7.3 to 7.6 in phosphate buffer, appears to require 0.15 M KCl for activity as determined by a DNA filter-binding assay, and is inhibited by N-ethylmaleimide. Like the untwisting enzymes from other eucaryotic cells, it can remove both positive and negative superhelical turns. A DNA molecule containing a single strand break was shown to be an intermediate in the untwisting reaction. Thermal stabilities of the enzyme from selected conditional lethal mutants defective in DNA synthesis have been examined and were found to be indistinguishable from the wild type enzyme.
J M Durnford, and
J J Champoux
September 1991,
FEBS letters,
J M Durnford, and
J J Champoux
October 1976,
Biochemistry,
J M Durnford, and
J J Champoux
May 1983,
FEBS letters,
J M Durnford, and
J J Champoux
April 1969,
Biochimica et biophysica acta,
J M Durnford, and
J J Champoux
May 1972,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
J M Durnford, and
J J Champoux
April 1983,
Antonie van Leeuwenhoek,
J M Durnford, and
J J Champoux
January 1988,
The Journal of biological chemistry,
J M Durnford, and
J J Champoux
October 1993,
The Journal of biological chemistry,
J M Durnford, and
J J Champoux
November 1985,
Proceedings of the National Academy of Sciences of the United States of America,
J M Durnford, and
J J Champoux
February 1992,
Archives of biochemistry and biophysics,
