BIOMAT Database Logo BIOMAT Database Logo

Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry. 2010

Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Department of Pediatrics, Duke University Medical Center, Durham NC 27709, USA.

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error=6%), precise (intra- and interday imprecision <8%), and sensitive (limit of detection=0.06pmol). Allantoin levels measured in control samples were comparable to literature values.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly

Related Publications

Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Quantification of Ephedrine Substances in Human Urine by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.
December 2022, Journal of chromatographic science,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Quantitation of Purine in Urine by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.
January 2022, Methods in molecular biology (Clifton, N.J.),
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Quantitation of Pyrimidine in Urine by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.
January 2022, Methods in molecular biology (Clifton, N.J.),
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
[Determination of perfluorinated compounds in human urine by ultra high performance liquid chromatography-tandem mass spectrometry].
February 2011, Se pu = Chinese journal of chromatography,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
[Determination of six bisphenols in human urine by ultra-high performance liquid chromatography-tandem mass spectrometry].
May 2024, Wei sheng yan jiu = Journal of hygiene research,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Determination of urine melamine by validated isotopic ultra-performance liquid chromatography/tandem mass spectrometry.
June 2009, Rapid communications in mass spectrometry : RCM,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Measurement of 1- and 3-methylhistidine in human urine by ultra performance liquid chromatography-tandem mass spectrometry.
January 2012, Clinica chimica acta; international journal of clinical chemistry,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Biomonitoring method for bisphenol A in human urine by ultra-high-performance liquid chromatography-tandem mass spectrometry.
March 2014, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Determination of Tranquilizers in Swine Urine by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry.
December 2018, Molecules (Basel, Switzerland),
Adviye A Tolun, and Haoyue Zhang, and Dora Il'yasova, and Judit Sztáray, and Sarah P Young, and David S Millington
Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra-performance Liquid Chromatography Tandem Mass Spectrometry.
January 2022, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!