The use of accelerometers is discussed to evaluate standing vertical jump. Two accelerometers, mounted on each ankle and connected to a wearable system, were used for signal acquisition, and a piezoelectric platform was used to verify the results. Fifty-one subjects were enrolled, subdivided into a group of healthy subjects and 2 groups who had different surgery for Achilles tendon rupture. Each subject performed 5 countermovement and 5 squat jumps; 11 subjects also performed 5 countermovement jumps with voluntary leg rotations during the flight phase. A training set was used to assess signal processing, and a validation set was used to verify its accuracy. A peak detection algorithm was developed to quantify flight time from the acceleration modulus, and its results were compared with platform data. The Pearson correlation coefficient of ankle accelerations and the integral of each signal were adopted to describe, respectively, the movement coordination and the limbs rotation during the flight time. The flight times obtained from the accelerometers and force plate were highly correlated (Spearman's coefficient >0.95); they were compared, for each jump, and the maximum mean error, for subject, was 4.8%. The movement coordination was in good agreement with subjects' clinical features and with the different jump phases. The signal integral presented significant differences, among jumps, related to leg rotations (p < 0.0005). The method proposed allows the monitoring of standing vertical jump using the fight time and gives information on the legs coordination and on the motor strategies of the lower limbs. Therefore, it can be used to obtain performance reference also outside labs, both in clinical and sport settings.