Visualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry. 2010

Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

The intraocular lens contains high levels of both cholesterol and sphingolipids, which are believed to be functionally important for normal lens physiology. The aim of this study was to explore the spatial distribution of sphingolipids in the ocular lens using mass spectrometry imaging (MSI). Matrix-assisted laser desorption/ionization (MALDI) imaging with ultra high resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) was used to visualize the lipid spatial distribution. Equatorially-cryosectioned, 12 microm thick slices of tissue were thaw-mounted to an indium-tin oxide (ITO) glass slide by soft-landing to an ethanol layer. This procedure maintained the tissue integrity. After the automated MALDI matrix deposition, the entire lens section was examined by MALDI MSI in a 150 microm raster. We obtained spatial- and concentration-dependent distributions of seven lens sphingomyelins (SM) and two ceramide-1-phosphates (CerP), which are important lipid second messengers. Glycosylated sphingolipids or sphingolipid breakdown products were not observed. Owing to ultra high resolution MS, all lipids were identified with high confidence, and distinct distribution patterns for each of them are presented. The distribution patterns of SMs provide an understanding of the physiological functioning of these lipids in clear lenses and offer a novel pathophysiological means for understanding diseases of the lens.

UI MeSH Term Description Entries
D007908 Lens, Crystalline A transparent, biconvex structure of the EYE, enclosed in a capsule and situated behind the IRIS and in front of the vitreous humor (VITREOUS BODY). It is slightly overlapped at its margin by the ciliary processes. Adaptation by the CILIARY BODY is crucial for OCULAR ACCOMMODATION. Eye Lens,Lens, Eye,Crystalline Lens
D005583 Fourier Analysis Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992) Fourier Series,Fourier Transform,Analysis, Cyclic,Analysis, Fourier,Cyclic Analysis,Analyses, Cyclic,Cyclic Analyses,Series, Fourier,Transform, Fourier
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013107 Sphingolipids A class of membrane lipids that have a polar head and two nonpolar tails. They are composed of one molecule of the long-chain amino alcohol sphingosine (4-sphingenine) or one of its derivatives, one molecule of a long-chain acid, a polar head alcohol and sometimes phosphoric acid in diester linkage at the polar head group. (Lehninger et al, Principles of Biochemistry, 2nd ed) Lysosphingolipids,Sphingolipid
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D050356 Lipid Metabolism Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS. Metabolism, Lipid
D053000 Analytic Sample Preparation Methods Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.
D057054 Molecular Imaging The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; FLUORESCENCE IMAGING; and MICROSCOPY. Imaging, Molecular
D019032 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. Laser Desorption-Ionization Mass Spectrometry, Matrix-Assisted,MALD-MS,MALDI,Mass Spectrometry, Matrix-Assisted Laser Desorption-Ionization,Mass Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry,Spectroscopy, Mass, Matrix-Assisted Laser Desorption-Ionization,MALDI-MS,MS-MALD,SELDI-TOF-MS,Surface Enhanced Laser Desorption Ionization Mass Spectrometry,Laser Desorption Ionization Mass Spectrometry, Matrix Assisted,MALDI MS,Mass Spectrometry, Matrix Assisted Laser Desorption Ionization,Mass Spectroscopy, Matrix Assisted Laser Desorption Ionization,Matrix Assisted Laser Desorption Ionization Mass Spectrometry

Related Publications

Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
January 2012, Acta biologica Hungarica,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
July 2013, Journal of the American Society for Mass Spectrometry,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
February 2022, Analytical chemistry,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
December 2022, Foods (Basel, Switzerland),
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
October 2022, Fitoterapia,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
September 2009, Investigative ophthalmology & visual science,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
October 2018, Journal of mass spectrometry : JMS,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
April 2009, Journal of lipid research,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
February 2018, Molecular pharmaceutics,
Veronika Vidová, and Jaroslav Pól, and Michael Volny, and Petr Novák, and Vladimír Havlícek, and Susanne K Wiedmer, and Juha M Holopainen
June 2016, Proteomics,
Copied contents to your clipboard!