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A radioimmunoassay for the measurement of both unconjugated and conjugaged estetrol in plasma has been developed. The antiserum obtained after 6 months of immunization with 6-oxoestetrol-6-(O-carboxy-methyl)oxim-BSA was used at a final dilution of 1:90,000 and showed almost no cross reaction with other steroids except for estriol at 1.24%. Esterol-glucosiduronate was synthesized by incubating with adrenalectomized rat liver homogenate and uridine diphosphoglucuronic acid. Then, plasma estetrol-glucosiduronate was measured in the same manner for unconjugated estetrol after hydrolysis with beta-glucuronidase. Sephadex LH-20 column chromatography (7X110 mm, benzene:methanol, 85:15) was employed for accurate assessment. The sensitivity was 10 pg and the smallest amount measurable was 40 pg/sample. The method bland was consistently negligible. The intra and inter assay precision was 11.8% and 14.2% for unconjugated estetrol and that for estetrol-glucosiduronate was 13.5% and 17.1%.
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
January 1979,
Hormone research,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
June 1979,
Acta medica Okayama,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
January 1982,
Clinical and experimental obstetrics & gynecology,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
April 1976,
Steroids,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
January 1974,
American journal of obstetrics and gynecology,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
September 1975,
The Journal of laboratory and clinical medicine,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
September 1974,
Steroids,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
February 1972,
Pediatric research,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
June 1979,
Acta Europaea fertilitatis,
K Den, and
H Matsumoto, and
K Fujii, and
K Furuya, and
T Yoshida, and
S Takagi, and
A Kanbegawa, and
T Kokubu
February 1978,
Nihon Naibunpi Gakkai zasshi,
