-transparent.png){kind=logo}
Monosodium urate monohydrate (MSUM) crystals derived from a tophus surgically removed from patients suffering from gout and MSUM prepared from a supersaturated solution of sodium urate were studied and compared with respect to their ability to: (1) stimulate chemiluminescence (CL) production by human polymorphonuclear (PMN) cells, (2) induce hemolysis of the human red blood cells and (3) induce inflammation when injected in the rat paw and knee joint. Human MSUM crystals were considerably more active in stimulating CL production by PMN cells and in inducing synovial inflammation. Both serum and papain pretreatment of human MSUM crystals caused inhibition of their enhancing effect on CL production by PMN cells. Papain pretreatment only reduced their phlogogenic activity. Uncoated and, to a much lesser extent, serum-coated human MSUM crystals induced secretion by mononuclear cells (MNC) of the factor(s) that considerably enhanced CL production by PMN cells. Both tophus-derived and synthetic crystals appeared to be weak hemolytic agents. Serum pretreatment of synthetic MSUM crystals reduced their hemolytic activity. These results suggest that surface coating, destroyed by papain treatment, was probably responsible for cell activation induced by human MSUM crystals.
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
May 1992,
The Journal of rheumatology,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
February 2019,
Chemical communications (Cambridge, England),
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
November 2002,
Arthritis and rheumatism,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
January 1980,
Advances in experimental medicine and biology,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
May 2000,
Current opinion in rheumatology,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
August 1968,
Yokohama medical bulletin,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
April 1976,
Journal of the American Chemical Society,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
January 1990,
Kidney international,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
October 1979,
The Journal of laboratory and clinical medicine,
A Stankovíc, and
P Front, and
A Barbara, and
D R Mitrovíc
August 1975,
Annals of the rheumatic diseases,
