Biomaterial Database

Enhanced isolation of influenza virus in conventional plate cell cultures by using low-speed centrifugation from clinical specimens. 1991

M Seno, and S Takao, and S Fukuda, and Y Kanamoto

Division of Microbiology, Hiroshima Prefectural Institute of Public Health, Japan.

To determine whether low-speed centrifugation as a means of amplifying viral adsorption to target cells can enhance the sensitivity of conventional techniques for the isolation of influenza virus from clinical specimens, the authors conducted a simultaneous comparison between two conventional plate cell cultures--one with and one without centrifugation (700 X g, 60 minutes). Of 26 influenza virus isolates obtained from 528 clinical specimens, 17 were more efficiently isolated by the centrifugation assay compared with conventional culture methods. Centrifugation-assisted methods were found to be efficient in the recovery of 17 of 20 isolates (85%) not identified on first passage by conventional techniques. The number of isolates obtained with centrifugation was 1.5 times as high as that obtained with no centrifugation after three passages. The two assays were significantly different in the isolation of influenza virus (P less than 0.01). Since low-speed centrifugation may increase the infectivity of influenza virus in cell culture, this technique may prove useful in the efficient and rapid isolation of this virus from clinical specimens.

UI	MeSH Term	Description	Entries
D008828	Microbiological Techniques	Techniques used in microbiology.	Microbiologic Technic,Microbiologic Technics,Microbiologic Technique,Microbiological Technics,Technic, Microbiologic,Technics, Microbiological,Technique, Microbiologic,Techniques, Microbiologic,Microbiologic Techniques,Microbiological Technic,Microbiological Technique,Technic, Microbiological,Technics, Microbiologic,Technique, Microbiological,Techniques, Microbiological
D009975	Orthomyxoviridae	A family of RNA viruses causing INFLUENZA and other respiratory diseases. Orthomyxoviridae includes INFLUENZAVIRUS A; INFLUENZAVIRUS B; INFLUENZAVIRUS C; INFLUENZAVIRUS D; ISAVIRUS; and THOGOTOVIRUS.	Influenza Viruses,Myxoviruses,Orthomyxoviruses,Influenza Virus,Myxovirus,Orthomyxovirus
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D005069	Evaluation Studies as Topic	Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies.	Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia