Biomaterial Database

Purification and properties of a nucleoside diphosphosugar: NAD+ 2-hexosyl oxidoreductase. 1978

M T Johnson, and J E Gander

Penicillium charlesii contains a nucleoside diphosphosugar: NAD 2-hexosyl oxidoreductase that oxidizes UDPgalactose (UDP-Galp), ADPribose [1,2] and UDPglucose (UDP-Glc). Dithiothreitol, NAD and 0.25 M NaCl but not nucleoside diphosphates stabilize the enzyme activity. The enzyme was purified and separated on polyacrylamide disc gels by electrophoresis into one major and eight minor bands of protein. Oxidoreductase activity was located in the major and three of the minor bands of protein. Each of these proteins catalyze the oxidation of UDPGalp, ADPribose and UDP-Glc.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D009243	NAD	A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed)	Coenzyme I,DPN,Diphosphopyridine Nucleotide,Nadide,Nicotinamide-Adenine Dinucleotide,Dihydronicotinamide Adenine Dinucleotide,NADH,Adenine Dinucleotide, Dihydronicotinamide,Dinucleotide, Dihydronicotinamide Adenine,Dinucleotide, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide,Nucleotide, Diphosphopyridine
D009702	Nucleoside Diphosphate Sugars		Diphosphate Sugars, Nucleoside,Sugars, Nucleoside Diphosphate
D010407	Penicillium	A mitosporic Trichocomaceae fungal genus that develops fruiting organs resembling a broom. When identified, teleomorphs include EUPENICILLIUM and TALAROMYCES. Several species (but especially PENICILLIUM CHRYSOGENUM) are sources of the antibiotic penicillin.	Penicilliums
D002237	Carbohydrate Dehydrogenases	Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.	Carbohydrate Oxidoreductases,Dehydrogenases, Carbohydrate,Oxidoreductases, Carbohydrate
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities