Molecular diversity of toxic components from the scorpion Heterometrus petersii venom revealed by proteomic and transcriptome analysis. 2010

Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.

Scorpion venoms contain a vast untapped reservoir of natural products, which have the potential for medicinal value in drug discovery. In this study, toxin components from the scorpion Heterometrus petersii venom were evaluated by transcriptome and proteome analysis.Ten known families of venom peptides and proteins were identified, which include: two families of potassium channel toxins, four families of antimicrobial and cytolytic peptides,and one family from each of the calcium channel toxins, La1-like peptides, phospholipase A2,and the serine proteases. In addition, we also identified 12 atypical families, which include the acid phosphatases, diuretic peptides, and ten orphan families. From the data presented here, the extreme diversity and convergence of toxic components in scorpion venom was uncovered. Our work demonstrates the power of combining transcriptomic and proteomic approaches in the study of animal venoms.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012604 Scorpion Venoms Venoms from animals of the order Scorpionida of the class Arachnida. They contain neuro- and hemotoxins, enzymes, and various other factors that may release acetylcholine and catecholamines from nerve endings. Of the several protein toxins that have been characterized, most are immunogenic. Scorpion Toxin,Scorpion Toxins,Scorpion Venom Peptide,Tityus serrulatus Venom,Scorpion Venom,alpha-Scorpion Toxin,beta-Scorpion Toxin,gamma-Scorpion Toxin,Peptide, Scorpion Venom,Toxin, Scorpion,Toxin, alpha-Scorpion,Toxin, beta-Scorpion,Venom Peptide, Scorpion,Venom, Scorpion,Venom, Tityus serrulatus,alpha Scorpion Toxin,beta Scorpion Toxin,gamma Scorpion Toxin
D012605 Scorpions Arthropods of the order Scorpiones, of which 1500 to 2000 species have been described. The most common live in tropical or subtropical areas. They are nocturnal and feed principally on insects and other arthropods. They are large arachnids but do not attack man spontaneously. They have a venomous sting. Their medical significance varies considerably and is dependent on their habits and venom potency rather than on their size. At most, the sting is equivalent to that of a hornet but certain species possess a highly toxic venom potentially fatal to humans. (From Dorland, 27th ed; Smith, Insects and Other Arthropods of Medical Importance, 1973, p417; Barnes, Invertebrate Zoology, 5th ed, p503) Scorpion
D016415 Sequence Alignment The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms. Sequence Homology Determination,Determination, Sequence Homology,Alignment, Sequence,Alignments, Sequence,Determinations, Sequence Homology,Sequence Alignments,Sequence Homology Determinations
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays
D040901 Proteomics The systematic study of the complete complement of proteins (PROTEOME) of organisms. Peptidomics

Related Publications

Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
March 2008, Proteomics,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
October 2015, Journal of proteomics,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
January 2011, Peptides,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
January 2014, PloS one,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
July 2010, BMC genomics,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
September 2018, Toxicon : official journal of the International Society on Toxinology,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
March 2018, Toxicon : official journal of the International Society on Toxinology,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
November 1978, Indian journal of experimental biology,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
September 2018, Toxins,
Yibao Ma, and Yong Zhao, and Ruiming Zhao, and Weiping Zhang, and Yawen He, and Yingliang Wu, and Zhijian Cao, and Lin Guo, and Wenxin Li
October 2017, Toxins,
Copied contents to your clipboard!