Biomaterial Database

The effect of pH on bilirubin binding by human erythrocytes. 1972

D Bratlid

Pediatric Research Institute, University Hospital, Rikshospitalet, Oslo, Norway.

The effect of pH on bilirubin binding by human erythrocytes was studied by incubating erythrocytes with solutions of bilirubin and albumin at pH levels 7.4-6.8. A reduction in pH from 7.4 greatly increased the cellular binding of bilirubin at all molar bilirubin/albumin ratios, even below the 1/1 limit. Spectrophotometric studies indicated that mainly the spectrum of the bilirubin molecule bound at the second binding site on the albumin molecule is affected by low pH. When cells were incubated with bilirubin without albumin, increased cellular binding of bilirubin at low pH was also found. However, the bilirubin concentrations greatly exceeded the solubility of bilirubin. The results and their possible clinical implications are discussed.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001663	Bilirubin	A bile pigment that is a degradation product of HEME.	Bilirubin IX alpha,Bilirubin, (15E)-Isomer,Bilirubin, (4E)-Isomer,Bilirubin, (4E,15E)-Isomer,Bilirubin, Calcium Salt,Bilirubin, Disodium Salt,Bilirubin, Monosodium Salt,Calcium Bilirubinate,Hematoidin,delta-Bilirubin,Bilirubinate, Calcium,Calcium Salt Bilirubin,Disodium Salt Bilirubin,Monosodium Salt Bilirubin,Salt Bilirubin, Calcium,delta Bilirubin
D012709	Serum Albumin	A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.	Plasma Albumin,Albumin, Serum
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.