Biomaterial Database

Production and extraction optimization of xylanase from Aspergillus niger DFR-5 through solid-state-fermentation. 2010

Ajay Pal, and Farhath Khanum

Biochemistry and Nutrition Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore, India. ajaydrdo@rediffmail.com

The effects of solid substrates, initial moisture content, moistening medium, temperature and incubation time on xylanase production by Aspergillus niger DFR-5 was studied and the highest activity (2596 IU/g dry substrate (gds)) was achieved in medium that contained wheat bran (WB) and soybean cake (SBC) at a ratio of 70:30, was moistened to 70% with MSS-2 mineral salt solution, and incubated for 6 days at 40 degrees C. Water at 37 degrees C was suitable for efficient recovery of enzyme from moldy WB-SBC medium. The extraction parameters for xylanase were optimized with respect to minimum volume of extractant using a central composite rotatable design (CCRD). The maximum recovery of xylanase (4465+/-52 IU/gds) with 92.5% desirability was obtained employing water (10 ml/gds) as extractant at 200 rpm for 60 min. The result shows that an overall 5.4-fold increase in xylanase production was obtained in concentrated form by optimizing medium components and extraction conditions.

UI	MeSH Term	Description	Entries
D007221	Industry	Any enterprise centered on the processing, assembly, production, or marketing of a line of products, services, commodities, or merchandise, in a particular field often named after its principal product. Examples include the automobile, fishing, music, publishing, insurance, and textile industries.	Tertiary Sector,Industries,Sector, Tertiary,Sectors, Tertiary,Tertiary Sectors
D007753	Laboratories	Facilities equipped to carry out investigative procedures.	Laboratory
D012044	Regression Analysis	Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable.	Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D005285	Fermentation	Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.	Fermentations
D000383	Agriculture	The science, art or practice of cultivating soil, producing crops, and raising livestock.	Agronomy,Agricultural Development,Farming,Agronomies,Development, Agricultural
D000704	Analysis of Variance	A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.	ANOVA,Analysis, Variance,Variance Analysis,Analyses, Variance,Variance Analyses
D001234	Aspergillus niger	An imperfect fungus causing smut or black mold of several fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food.	Aspergillus lacticoffeatus
D001709	Biotechnology	Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.	Biotechnologies
D012997	Solvents	Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)	Solvent
D013696	Temperature	The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.	Temperatures