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We established specific and highly sensitive radioimmunoassay (RIA) for the detection of human brain natriuretic peptide (BNP), and confirmed the presence of BNP-like immunoreactivity (BNP-LI) in human circulating blood and the heart. The antibody used in this RIA did not react any known human atrial natriuretic peptide (ANP). Gel permeation chromatography coupled with the RIA revealed that BNP-LI in atrium is composed with high molecular BNP and low molecular BNP. Furthermore, dominant form of BNP-LI in human plasma was thought to be low molecular BNP. Mean concentration of BNP-LI in plasma from normal subjects was 5.9 pg/ml, which was about 5 times lower than that of ANP-LI. Mean concentration of BNP-LI in human cardiac atrium was 45.6 ng/mg protein, which was about 40-fold lower than that of ANP-LI. Furthermore, concentration of BNP-LI in human cardiac ventricle was in a range of 0.1-4.8 ng/mg protein, being about 1/20 that of cardiac atrium. These data suggest that BNP synthesized in human heart and secreted into circulation.
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
May 1986,
Biochemical and biophysical research communications,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
April 1993,
International journal of pancreatology : official journal of the International Association of Pancreatology,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
January 1988,
Zeitschrift fur Kardiologie,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
April 1988,
Clinical chemistry,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
March 1988,
Journal of pharmaceutical sciences,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
March 1995,
Nihon rinsho. Japanese journal of clinical medicine,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
January 1983,
Zeitschrift fur Naturforschung. Section C, Biosciences,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
July 1986,
The Journal of clinical endocrinology and metabolism,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
January 1989,
Biochemical and biophysical research communications,
K Togashi, and
K Ando, and
T Kameya, and
M Kawakami
June 1987,
Clinica chimica acta; international journal of clinical chemistry,
