Protective efficacy of the H5 inactivated vaccine against different highly pathogenic H5N1 avian influenza viruses isolated in China and Vietnam. 2010

Guobin Tian, and Xianying Zeng, and Yanbing Li, and Jianzhong Shi, and Hualan Chen
Animal Influenza Laboratory of the Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People's Republic of China.

An inactivated H5N1 avian influenza (AI) vaccine generated by reverse genetics and containing the hemagglutinin and neuraminidase genes of the H5N1 A/goose/Guangdong/1/96 (GS/GD/06) virus has been used in domestic poultry in China and Vietnam as an important control strategy for H5N1 AI. The efficacy of this vaccine against early H5N1 isolates has been fully evaluated in chicken, ducks, and geese. However, there are no reports about its efficacy against H5N1 viruses recently isolated in China and Vietnam. In this study, groups of 3-wk-old specific-pathogen-free chickens were intramuscularly injected with one dose of the vaccine. Three weeks postvaccination, the chickens were intranasally challenged with 10(5)EID50 of six different lethal H5N1 AI viruses: A/bar-headed goose/Qinghai/3/05 (clade 2.2), A/chicken/Shanxi/2/06 (CK/SX/06; clade 7), A/duck/Fujian/31/07 (clade 2.3.4), A/MDK/VN-HD/46/07 (clade 2.3.4), A/MDK/VN-CM/1185/06 (clade 1), and A/MDK/VN-CM/1159/06 (clade 1). Four out of 20 chickens challenged with the CK/SX/06 shed virus on day 5 and died on day 8 to 9 postchallenge. Chickens challenged with the remaining five viruses were completely protected (no disease signs, virus shedding, or deaths). These results indicate that the GS/GD/06-based vaccine provides sound protection against clade 1, 2.2, and 2.3.4 viruses, but not against the CK/SX/06 virus, which emerged in northern China in 2006.

UI MeSH Term Description Entries
D007252 Influenza Vaccines Vaccines used to prevent infection by viruses in the family ORTHOMYXOVIRIDAE. It includes both killed and attenuated vaccines. The composition of the vaccines is changed each year in response to antigenic shifts and changes in prevalence of influenza virus strains. The flu vaccines may be mono- or multi-valent, which contains one or more ALPHAINFLUENZAVIRUS and BETAINFLUENZAVIRUS strains. Flu Vaccine,Influenzavirus Vaccine,Monovalent Influenza Vaccine,Universal Flu Vaccine,Universal Influenza Vaccine,Flu Vaccines,High-Dose Trivalent Influenza Vaccine,Influenza Vaccine,Influenza Virus Vaccine,Influenza Virus Vaccines,Influenzavirus Vaccines,Intranasal Live-Attenuated Influenza Vaccine,LAIV Vaccine,Monovalent Influenza Vaccines,Quadrivalent Influenza Vaccine,Trivalent Influenza Vaccine,Trivalent Live Attenuated Influenza Vaccine,Universal Flu Vaccines,Universal Influenza Vaccines,Flu Vaccine, Universal,High Dose Trivalent Influenza Vaccine,Influenza Vaccine, Monovalent,Influenza Vaccine, Quadrivalent,Influenza Vaccine, Trivalent,Influenza Vaccine, Universal,Intranasal Live Attenuated Influenza Vaccine,Vaccine, Flu,Vaccine, Influenza,Vaccine, Influenza Virus,Vaccine, Influenzavirus,Vaccine, LAIV,Vaccine, Monovalent Influenza,Vaccine, Quadrivalent Influenza,Vaccine, Trivalent Influenza,Virus Vaccine, Influenza
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D002681 China A country spanning from central Asia to the Pacific Ocean. Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D005585 Influenza in Birds Infection of domestic and wild fowl and other BIRDS with INFLUENZA A VIRUS. Avian influenza usually does not sicken birds, but can be highly pathogenic and fatal in domestic POULTRY. Avian Flu,Avian Influenza,Fowl Plague,Influenza, Avian,Avian Influenzas,Flu, Avian,Influenza in Bird,Influenzas, Avian,Plague, Fowl
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D013047 Specific Pathogen-Free Organisms Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free. Pathogen-Free Organisms,Specific Pathogen Free,Organism, Pathogen-Free,Organism, Specific Pathogen-Free,Organisms, Pathogen-Free,Organisms, Specific Pathogen-Free,Pathogen Free Organisms,Pathogen Free, Specific,Pathogen Frees, Specific,Pathogen-Free Organism,Pathogen-Free Organism, Specific,Pathogen-Free Organisms, Specific,Specific Pathogen Free Organisms,Specific Pathogen-Free Organism
D014744 Vietnam A country in southeastern Asia, bordering the Gulf of Thailand, Gulf of Tonkin, and South China Sea, as well as China, Laos, and Cambodia. The capital is Hanoi. North Vietnam,Viet Nam,Vietnam, Republic of
D015164 Vaccines, Inactivated Vaccines in which the infectious microbial nucleic acid components have been destroyed by chemical or physical treatment (e.g., formalin, beta-propiolactone, gamma radiation) without affecting the antigenicity or immunogenicity of the viral coat or bacterial outer membrane proteins. Inactivated Vaccine,Killed Vaccine,Killed Vaccines,Vaccines, Killed,Inactivated Vaccines,Vaccine, Inactivated,Vaccine, Killed
D053124 Influenza A Virus, H5N1 Subtype A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 5 and neuraminidase 1. The H5N1 subtype, frequently referred to as the bird flu virus, is endemic in wild birds and very contagious among both domestic (POULTRY) and wild birds. It does not usually infect humans, but some cases have been reported. H5N1 Avian Influenza Virus,H5N1 Bird Flu Virus,H5N1 Virus,H5N1 subtype,H5N1 Viruses,H5N1 subtypes,Virus, H5N1

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