BIOMAT Database Logo BIOMAT Database Logo

Parthenogenic blastocysts derived from cumulus-free in vitro matured human oocytes. 2010

Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, Palo Alto, California, United States of America.

BACKGROUND Approximately 20% of oocytes are classified as immature and discarded following intracytoplasmic sperm injection (ICSI) procedures. These oocytes are obtained from gonadotropin-stimulated patients, and are routinely removed from the cumulus cells which normally would mature the oocytes. Given the ready access to these human oocytes, they represent a potential resource for both clinical and basic science application. However culture conditions for the maturation of cumulus-free oocytes have not been optimized. We aimed to improve maturation conditions for cumulus-free oocytes via culture with ovarian paracrine/autocrine factors identified by single cell analysis. RESULTS Immature human oocytes were matured in vitro via supplementation with ovarian paracrine/autocrine factors that were selected based on expression of ligands in the cumulus cells and their corresponding receptors in oocytes. Matured oocytes were artificially activated to assess developmental competence. Gene expression profiles of parthenotes were compared to IVF/ICSI embryos at morula and blastocyst stages. Following incubation in medium supplemented with ovarian factors (BDNF, IGF-I, estradiol, GDNF, FGF2 and leptin), a greater percentage of oocytes demonstrated nuclear maturation and subsequently, underwent parthenogenesis relative to control. Similarly, cytoplasmic maturation was also improved as indicated by development to blastocyst stage. Parthenogenic blastocysts exhibited mRNA expression profiles similar to those of blastocysts obtained after IVF/ICSI with the exception for MKLP2 and PEG1. CONCLUSIONS Human cumulus-free oocytes from hormone-stimulated cycles are capable of developing to blastocysts when cultured with ovarian factor supplementation. Our improved IVM culture conditions may be used for obtaining mature oocytes for clinical purposes and/or for derivation of embryonic stem cells following parthenogenesis or nuclear transfer.

UI MeSH Term Description Entries
D009865 Oocytes Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM). Ovocytes,Oocyte,Ovocyte
D010053 Ovary The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE. Ovaries
D010312 Parthenogenesis A unisexual reproduction without the fusion of a male and a female gamete (FERTILIZATION). In parthenogenesis, an individual is formed from an unfertilized OVUM that did not complete MEIOSIS. Parthenogenesis occurs in nature and can be artificially induced. Arrhenotoky,Automixis,Thelytoky,Parthenogeneses
D001755 Blastocyst A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper. Embryo, Preimplantation,Blastocysts,Embryos, Preimplantation,Preimplantation Embryo,Preimplantation Embryos
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D054885 Cumulus Cells The granulosa cells of the cumulus oophorus which surround the OVUM in the GRAAFIAN FOLLICLE. At OVULATION they are extruded with OVUM. Granulosa Cells, Cumulus,Cumulus Granulosa Cells,Cell, Cumulus,Cell, Cumulus Granulosa,Cells, Cumulus,Cells, Cumulus Granulosa,Cumulus Cell,Cumulus Granulosa Cell,Granulosa Cell, Cumulus
D020869 Gene Expression Profiling The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell. Gene Expression Analysis,Gene Expression Pattern Analysis,Transcript Expression Analysis,Transcriptome Profiling,Transcriptomics,mRNA Differential Display,Gene Expression Monitoring,Transcriptome Analysis,Analyses, Gene Expression,Analyses, Transcript Expression,Analyses, Transcriptome,Analysis, Gene Expression,Analysis, Transcript Expression,Analysis, Transcriptome,Differential Display, mRNA,Differential Displays, mRNA,Expression Analyses, Gene,Expression Analysis, Gene,Gene Expression Analyses,Gene Expression Monitorings,Gene Expression Profilings,Monitoring, Gene Expression,Monitorings, Gene Expression,Profiling, Gene Expression,Profiling, Transcriptome,Profilings, Gene Expression,Profilings, Transcriptome,Transcript Expression Analyses,Transcriptome Analyses,Transcriptome Profilings,mRNA Differential Displays
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Pregnancies established from water buffalo (Bubalus bubalis) blastocysts derived from in vitro matured, in vitro fertilized oocytes and co-cultured with cumulus and oviductal cells.
September 1994, Theriogenology,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Pregnancies and deliveries after transfer of human blastocysts derived from in vitro matured oocytes in in vitro maturation cycles.
June 2007, Fertility and sterility,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Parthenogenic development of in vitro-matured, in vivo-cultured porcine oocytes beyond blastocyst.
February 1997, Biology of reproduction,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Parthenogenic activation of surplus in vitro-matured human oocytes: a tool for validation of oocyte cryopreservation.
December 2009, Fertility and sterility,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Effect of vitrification on the expression of genes in porcine blastocysts derived from in vitro matured oocytes.
August 2022, Systems biology in reproductive medicine,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Effects of cumulus cells on culture of bovine embryos derived from oocytes matured and fertilized in vitro.
September 1993, Journal of animal science,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Gene expression profiles in mouse cumulus cells derived from in vitro matured oocytes with and without blastocyst formation.
November 2017, Gene expression patterns : GEP,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Production and lambing rate of blastocysts derived from in vitro matured oocytes after gonadotropin treatment of prepubertal ewes.
August 1999, Journal of animal science,
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Timing of in-vitro fertilization of cumulus-free and cumulus-enclosed human oocytes.
June 1986, Human reproduction (Oxford, England),
Sohyun L McElroy, and James A Byrne, and Shawn L Chavez, and Barry Behr, and Aaron J Hsueh, and Lynn M Westphal, and Renee A Reijo Pera
Pregnancies after co-culture of cumulus cells with bovine embryos derived from in-vitro fertilization of in-vitro matured follicular oocytes.
July 1988, Journal of reproduction and fertility,
Copied contents to your clipboard!