Characterization of two human lung adenocarcinoma cell lines by reciprocal chromosome painting. 2010

Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China.

Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified in A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines.

UI MeSH Term Description Entries
D007621 Karyotyping Mapping of the KARYOTYPE of a cell. Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002869 Chromosome Aberrations Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS. Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D002871 Chromosome Banding Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping. Banding, Chromosome,Bandings, Chromosome,Chromosome Bandings
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000034 Abortion, Veterinary Premature expulsion of the FETUS in animals. Veterinary Abortion,Abortions, Veterinary,Veterinary Abortions
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017404 In Situ Hybridization, Fluorescence A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei. FISH Technique,Fluorescent in Situ Hybridization,Hybridization in Situ, Fluorescence,FISH Technic,Hybridization in Situ, Fluorescent,In Situ Hybridization, Fluorescent,FISH Technics,FISH Techniques,Technic, FISH,Technics, FISH,Technique, FISH,Techniques, FISH
D020223 Chromosome Painting A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species. Chromosome Paintings,Painting, Chromosome,Paintings, Chromosome

Related Publications

Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
May 2000, Cancer genetics and cytogenetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
July 1997, Cancer genetics and cytogenetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
January 1997, Cytogenetics and cell genetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
August 2000, Cancer genetics and cytogenetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
August 1992, American journal of respiratory cell and molecular biology,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
January 1994, Anticancer research,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
December 1998, Cancer genetics and cytogenetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
January 1999, Cytogenetics and cell genetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
January 1995, American journal of medical genetics,
Kun-Jing Peng, and Jin-Huan Wang, and Wei-Ting Su, and Xi-Cai Wang, and Feng-Tang Yang, and Wen-Hui Nie
February 1993, Bollettino della Societa italiana di biologia sperimentale,
Copied contents to your clipboard!