Biomaterial Database

Biodegradation characteristics of quinoline by Pseudomonas putida. 2010

Qiao Lin, and Wang Jianlong

Laboratory of Environmental Technology, INET, Tsinghua University, Beijing, PR China.

A quinoline-degrading strain was isolated from activated sludge of the municipal wastewater treatment plant and identified as Pseudomonas putida on the basis of its partial 16S rRNA gene sequence analysis. The characteristics of quinoline degradation were investigated. The experimental results showed that quinoline at the concentration of 500 mg/L could be completely removed within 3h, but could not be mineralized because special pigments were observed in the process of quinoline biodegradation. The N-atom in the quinoline ring was released as NH(3); some metabolic intermediates were identified, including 2-hydroxyquinoline, 2,8-dihydroxyquinoline and 8-hydroxycoumarin. The metabolic pathway of quinoline by P. putida was tentatively proposed, that is, via 8-hydroxycoumarin pathway, namely, the biodegradation was initiated by hydroxylation at position 2 to form 2-oxo-1,2-dihydroquinoline (2-hydroxyquinoline), which was then oxidized to 8-hydroxy-2-oxo-1,2-dihydro-quinoline (2,8-dihydroxyquinoline) and 8-hydroxycoumarin.

UI	MeSH Term	Description	Entries
D009584	Nitrogen	An element with the atomic symbol N, atomic number 7, and atomic weight [14.00643; 14.00728]. Nitrogen exists as a diatomic gas and makes up about 78% of the earth's atmosphere by volume. It is a constituent of proteins and nucleic acids and found in all living cells.
D011725	Pyridines	Compounds with a six membered aromatic ring containing NITROGEN. The saturated version is PIPERIDINES.
D011804	Quinolines
D000641	Ammonia	A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
D001673	Biodegradation, Environmental	Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.	Bioremediation,Phytoremediation,Natural Attenuation, Pollution,Environmental Biodegradation,Pollution Natural Attenuation
D016958	Pseudomonas putida	A species of gram-negative, aerobic bacteria isolated from soil and water as well as clinical specimens. Occasionally it is an opportunistic pathogen.