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Fixation methods for electron microscopy of human and other liver. 2010
Eddie Wisse, and
Filip Braet, and
Hans Duimel, and
Celien Vreuls, and
Ger Koek, and
Steven W M Olde Damink, and
Maartje A J van den Broek, and
Bart De Geest, and
Cees H C Dejong, and
Chise Tateno, and
Peter Frederik
Electron Microscope Unit, University of Maastricht, 6200 MD Maastricht, The Netherlands. eddie@wisse.be
For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects.
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