Biomaterial Database

Role of host lysosomal associated membrane protein (LAMP) in Trypanosoma cruzi invasion and intracellular development. 2010

L A G Albertti, and A M Macedo, and E Chiari, and N W Andrews, and L O Andrade

Department of Morphology, Federal University of Minas Gerais, Antonio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil.

Trypanosoma cruzi host cell entry depends on lysosomes for the formation of the parasitophorous vacuole. Lysosome internal surface is covered by two major proteins, highly sialilated, Lysosome Associated Membrane Proteins 1 and 2. T. cruzi, on the other hand, needs to acquire sialic acid from its host cell through the activity of trans-sialidase, an event that contributes to host cell invasion and later for parasite vacuole escape. Using LAMP1/2 knock out cells we were able to show that these two proteins are important for T. cruzi infection of host cells, both in entrance and intracellular development, conceivably by being the major source of sialic acid for T. cruzi.

UI	MeSH Term	Description	Entries
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014349	Trypanosoma cruzi	The agent of South American trypanosomiasis or CHAGAS DISEASE. Its vertebrate hosts are man and various domestic and wild animals. Insects of several species are vectors.	Trypanosoma cruzus,cruzi, Trypanosoma
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D051907	Lysosomal Membrane Proteins	Ubiquitously expressed integral membrane glycoproteins found in the LYSOSOME.	LAMP Lysosomal-Associated Membrane Protein,LAMP Lysosomal-Associated Membrane Proteins,Lysosomal Integral Membrane Protein,Lysosomal Membrane Glycoprotein,Lysosomal Membrane Protein,Lysosomal-Associated Membrane Protein,Lysosome-Associated Membrane Glycoprotein,Lysosome-Associated Membrane Glycoproteins,Lysosome-Associated Membrane Protein,Lysosomal Integral Membrane Proteins,Lysosomal Membrane Glycoproteins,Lysosomal-Associated Membrane Proteins,Lysosome-Associated Membrane Proteins,LAMP Lysosomal Associated Membrane Protein,LAMP Lysosomal Associated Membrane Proteins,Lysosomal Associated Membrane Protein,Lysosomal Associated Membrane Proteins,Lysosome Associated Membrane Glycoprotein,Lysosome Associated Membrane Glycoproteins,Lysosome Associated Membrane Protein,Lysosome Associated Membrane Proteins,Membrane Glycoprotein, Lysosomal,Membrane Glycoprotein, Lysosome-Associated,Membrane Glycoproteins, Lysosomal,Membrane Glycoproteins, Lysosome-Associated,Membrane Protein, Lysosomal,Membrane Protein, Lysosomal-Associated,Membrane Protein, Lysosome-Associated,Membrane Proteins, Lysosomal,Membrane Proteins, Lysosomal-Associated,Membrane Proteins, Lysosome-Associated
D052119	Lysosomal-Associated Membrane Protein 2	An abundant lysosomal-associated membrane protein that has been found to shuttle between LYSOSOMES; ENDOSOMES; and the PLASMA MEMBRANE. Loss of expression of lysosomal-associated membrane protein 2 is associated with GLYCOGEN STORAGE DISEASE TYPE IIB.	Antigens, CD107b,CD107b Antigens,CD107b Antigen,LAMP2 Protein,LGP-B Protein,LGP110 Protein,LGP B Protein,Lysosomal Associated Membrane Protein 2
D054884	Host-Pathogen Interactions	The interactions between a host and a pathogen, usually resulting in disease.	Host Pathogen Interaction,Host-Pathogen Relations,Pathogen-Host Interaction,Pathogen-Host Interactions,Host Pathogen Interactions,Host Pathogen Relations,Host-Pathogen Interaction,Host-Pathogen Relation,Interaction, Host Pathogen,Interaction, Host-Pathogen,Interaction, Pathogen-Host,Interactions, Host Pathogen,Interactions, Host-Pathogen,Interactions, Pathogen-Host,Pathogen Host Interaction,Pathogen Host Interactions,Pathogen Interaction, Host,Pathogen Interactions, Host,Relation, Host-Pathogen,Relations, Host-Pathogen
D018345	Mice, Knockout	Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.	Knockout Mice,Mice, Knock-out,Mouse, Knockout,Knock-out Mice,Knockout Mouse,Mice, Knock out