Enhanced 3-D reconstruction from confocal scanning microscope images. 1: Deterministic and maximum likelihood reconstructions. 1990

J A Conchello, and E W Hansen

Significant improvement in the longitudinal (optical axis) resolution of a microscope has previously been obtained either by posterior digital processing of optical sections collected with a conventional microscope, or by collecting sections with a confocal scanning microscope. In this paper we report the feasibility of obtaining longitudinal resolution comparable to the lateral diffraction limit by posterior processing of confocal sections. A confocal through-focus image series was simulated numerically and restored by constrained iterative deconvolution and by maximum likelihood. Several typical imaging situations were simulated. The results support the possibility of achieving equal longitudinal and lateral resolution.

UI MeSH Term Description Entries

Related Publications

J A Conchello, and E W Hansen
November 2001, IEEE transactions on bio-medical engineering,
J A Conchello, and E W Hansen
July 2011, IEEE transactions on image processing : a publication of the IEEE Signal Processing Society,
J A Conchello, and E W Hansen
August 2005, Current protocols in neuroscience,
J A Conchello, and E W Hansen
April 2014, Current protocols in neuroscience,
J A Conchello, and E W Hansen
January 1999, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society,
Copied contents to your clipboard!