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The computed tomography (CT) technique is implemented in an optical transmission microscope with a laser as a light source. A rotational Pechan prism is used in the illumination optics of the microscope for projecting a thick 3-D sample onto a 2-D array detector in different directions. A minicomputer is employed for 3-D reconstruction from the observed image data. Energy throughput efficiency and signal-to-noise ratio in the developed CT microscope are higher than those of our previously developed CT microscope [J. Opt. Soc. Am. A 4, 292 (1987)]. Instrumentation of this microscope is described and some experimental results are shown with biological samples.
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