BIOMAT Database Logo BIOMAT Database Logo

Rat monoclonal antibody specific for MyoD. 2010

Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Myogenic determination 1 (MyoD) is a myogenic regulatory factor (MRF) possessing a basic domain and a helix-loop-helix domain. MRFs play a critical role in myoblast fate and terminal differentiation. MyoD is a transcriptional factor that induces transcription by binding with gene regulatory factors expressed in skeletal muscle. As a master gene, MyoD also determines skeletal muscle differentiation. In this study, we established a monoclonal antibody specific for MyoD using the rat medial iliac lymph node method. Immunoblot analysis revealed that our monoclonal antibody against MyoD could identify full-length MyoD. Moreover, immunocytochemical staining revealed a change in the expression of MyoD at the skeletal muscle differentiation stage. This monoclonal antibody against MyoD allows for further studies to elucidate the mechanism by which MyoD influences skeletal muscle differentiation.

UI MeSH Term Description Entries
D007082 Ileum The distal and narrowest portion of the SMALL INTESTINE, between the JEJUNUM and the ILEOCECAL VALVE of the LARGE INTESTINE.
D007114 Immunization Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow). Immunologic Stimulation,Immunostimulation,Sensitization, Immunologic,Variolation,Immunologic Sensitization,Immunological Stimulation,Sensitization, Immunological,Stimulation, Immunologic,Immunizations,Immunological Sensitization,Immunological Sensitizations,Immunological Stimulations,Sensitizations, Immunological,Stimulation, Immunological,Stimulations, Immunological,Variolations
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D011921 Rats, Inbred WKY A strain of Rattus norvegicus used as a normotensive control for the spontaneous hypertensive rats (SHR). Rats, Wistar Kyoto,Wistar Kyoto Rat,Rats, WKY,Inbred WKY Rat,Inbred WKY Rats,Kyoto Rat, Wistar,Rat, Inbred WKY,Rat, WKY,Rat, Wistar Kyoto,WKY Rat,WKY Rat, Inbred,WKY Rats,WKY Rats, Inbred,Wistar Kyoto Rats
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002467 Cell Nucleus Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed) Cell Nuclei,Nuclei, Cell,Nucleus, Cell
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006825 Hybridomas Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell. Hybridoma

Related Publications

Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Rat monoclonal antibody specific for septin 9.
April 2010, Hybridoma (2005),
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
A monoclonal antibody specific for rat intestinal lymphocytes.
January 1986, Journal of immunology (Baltimore, Md. : 1950),
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Rat monoclonal antibody specific for prostaglandin E structure.
August 1985, Biochimica et biophysica acta,
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Generation of rat monoclonal antibody specific for mouse importin α8.
June 2011, Hybridoma (2005),
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Generation of a rat monoclonal antibody specific for glyoxalase I.
December 2009, Hybridoma (2005),
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
A rat monoclonal antibody specific for murine type 1 pneumocytes.
December 1992, Experimental and molecular pathology,
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Monoclonal antibody specific for lactosylceramide.
May 1984, The Journal of biological chemistry,
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Generation and characterization of a rat monoclonal antibody specific for PCNA.
April 2008, Hybridoma (2005),
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.
December 2003, Hybridoma and hybridomics,
Akihito Harada, and Yasuyuki Ohkawa, and Shinpei Ao, and Jun Odawara, and Seiji Okada, and Masayuki Azuma, and Yuko Nishiyama, and Mako Nakamura, and Taro Tachibana
Binding site of the rat liver specific monoclonal antibody.
May 1988, Biochemical and biophysical research communications,
Copied contents to your clipboard!