A rapid protocol to isolate DNA fragments from low melting temperature agarose.
1991
R S Seelan, and
L I Grossman
Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201.
UI
MeSH Term
Description
Entries
D004247
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D004587
Electrophoresis, Agar Gel
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D005821
Genetic Techniques
Chromosomal, biochemical, intracellular, and other methods used in the study of genetics.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.