Biomaterial Database

Effect of restraint stress on 2,3,7,8 tetrachloro dibenzo-p-dioxin induced testicular and epididymal toxicity in rats. 2011

S Dhanabalan, and R Jubendradass, and P Latha, and P P Mathur

Department of Biochemistry and Molecular Biology, School of Life Sciences, Pondicherry University, Pondicherry, India.

Dioxins like 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) impair male reproductive system by increasing the generation of reactive oxygen species (ROS). Glucocorticoids have been found to suppress male reproductive function and also influence TCDD pathway. As stress is characterized by an increase in the level and activity of glucocorticoids, the present experiments were conducted to evaluate the effect of restraint stress on TCDD-induced testicular and epididymal toxicity. Adult male Wistar rats were subjected to either restraint stress (5 hours/day) or TCDD treatment (100 ng/kg b.w./day) or both for 15 days. Restraint stress or TCDD treatment raised the serum level of corticosterone and suppressed the testicular level of steroidogenic acute regulatory (StAR) protein and serum level of testosterone significantly. In the testis and epididymis, restraint stress or TCDD treatment raised the levels of lipid peroxidation and hydrogen peroxide and suppressed the activities of antioxidant enzymes significantly. In rats subjected to both restraint stress and TCDD treatment, a significant increase in the serum level of corticosterone and a significant decrease in the testicular level of StAR protein and serum level of testosterone were observed as compared to rats treated with TCDD alone. A significant increase in the levels of lipid peroxidation and hydrogen peroxide and a significant decrease in the activities of antioxidant enzymes were observed in the testis and epididymis of rats subjected to both restraint stress and TCDD treatment as compared to TCDD alone treated rats. Thus, restraint stress potentiates the adverse effects of TCDD on male reproductive organs.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D010750	Phosphoproteins		Phosphoprotein
D012149	Restraint, Physical	Use of a device for the purpose of controlling movement of all or part of the body. Splinting and casting are FRACTURE FIXATION.	Immobilization, Physical,Physical Restraint,Physical Immobilization,Physical Restraints,Restraints, Physical
D003345	Corticosterone	An adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. (From Goodman and Gilman's The Pharmacological Basis of Therapeutics, 8th ed, p1437)
D004785	Environmental Pollutants	Substances or energies, for example heat or light, which when introduced into the air, water, or land threaten life or health of individuals or ECOSYSTEMS.	Environmental Pollutant,Pollutant,Pollutants,Pollutants, Environmental,Pollutant, Environmental
D004822	Epididymis	The convoluted cordlike structure attached to the posterior of the TESTIS. Epididymis consists of the head (caput), the body (corpus), and the tail (cauda). A network of ducts leaving the testis joins into a common epididymal tubule proper which provides the transport, storage, and maturation of SPERMATOZOA.
D006861	Hydrogen Peroxide	A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.	Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D000072317	Polychlorinated Dibenzodioxins	Dibenzodioxin derivatives that contain multiple chloride atoms bound to the benzene ring structures.	TCDD,Tetrachlorodibenzodioxin,2,3,7,8-Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo-p-dioxins,Dibenzo(b,e)(1,4)dioxin, 2,3,7,8-tetrachloro-,PCDD,Polychlorinated Dibenzo-p-dioxins,Polychlorinated Dibenzodioxin,Polychlorodibenzo-4-dioxin,Polychlorodibenzo-p-dioxin,Tetrachlorodibenzo-p-dioxin,Chlorinated Dibenzo p dioxins,Dibenzo-p-dioxins, Chlorinated,Dibenzo-p-dioxins, Polychlorinated,Dibenzodioxin, Polychlorinated,Dibenzodioxins, Polychlorinated,Polychlorinated Dibenzo p dioxins,Polychlorodibenzo 4 dioxin,Polychlorodibenzo p dioxin,Tetrachlorodibenzo p dioxin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia