Increased UV-induced sister-chromatid exchange in cultured fibroblasts of first-degree relatives of melanoma patients. 1991

S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
Department of Occupational Medicine, University of Hamburg, West Germany.

Cultured fibroblasts of 17 first-degree relatives of familial melanoma patients and six first-degree relatives of cutaneous melanoma (CMM) patients with multiple CMM primaries were tested for in vitro sensitivity to UV light. Fibroblasts of nine familial CMM patients with a known UV-sensitivity and 19 healthy probands served as a control. Sister chromatid exchange (SCE) was used as a parameter to detect UV-induced genotoxic damage. We found significantly (p less than 0.001) increased UV-induced SCE levels in familial melanoma patients, as well as in first-degree relatives of familial melanoma patients (p less than 0.001) after UV-A,B irradiation (375 J/m2), compared to the healthy probands without a family history of CMM. A significant (p less than 0.001) increase of UV-induced SCE was also observed in the relatives of CMM patients with multiple CMM primaries. In addition, the spontaneous SCE were significantly increased (p less than 0.05) in familial CMM patients. This study shows that increased UV sensitivity is a familial phenomenon. It is consistent with the concept of a genetic predisposition to CMM, which is based on increased UV sensitivity and may help to define groups with an elevated risk of developing cutaneous malignant melanoma.

UI MeSH Term Description Entries
D008545 Melanoma A malignant neoplasm derived from cells that are capable of forming melanin, which may occur in the skin of any part of the body, in the eye, or, rarely, in the mucous membranes of the genitalia, anus, oral cavity, or other sites. It occurs mostly in adults and may originate de novo or from a pigmented nevus or malignant lentigo. Melanomas frequently metastasize widely, and the regional lymph nodes, liver, lungs, and brain are likely to be involved. The incidence of malignant skin melanomas is rising rapidly in all parts of the world. (Stedman, 25th ed; from Rook et al., Textbook of Dermatology, 4th ed, p2445) Malignant Melanoma,Malignant Melanomas,Melanoma, Malignant,Melanomas,Melanomas, Malignant
D011836 Radiation Tolerance The ability of some cells or tissues to survive lethal doses of IONIZING RADIATION. Tolerance depends on the species, cell type, and physical and chemical variables, including RADIATION-PROTECTIVE AGENTS and RADIATION-SENSITIZING AGENTS. Radiation Sensitivity,Radiosensitivity,Sensitivity, Radiation,Tolerance, Radiation,Radiation Sensitivities,Radiation Tolerances,Radiosensitivities,Sensitivities, Radiation,Tolerances, Radiation
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012854 Sister Chromatid Exchange An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME. Chromatid Exchange, Sister,Chromatid Exchanges, Sister,Exchange, Sister Chromatid,Exchanges, Sister Chromatid,Sister Chromatid Exchanges
D014466 Ultraviolet Rays That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants. Actinic Rays,Black Light, Ultraviolet,UV Light,UV Radiation,Ultra-Violet Rays,Ultraviolet Light,Ultraviolet Radiation,Actinic Ray,Light, UV,Light, Ultraviolet,Radiation, UV,Radiation, Ultraviolet,Ray, Actinic,Ray, Ultra-Violet,Ray, Ultraviolet,Ultra Violet Rays,Ultra-Violet Ray,Ultraviolet Black Light,Ultraviolet Black Lights,Ultraviolet Radiations,Ultraviolet Ray
D066298 In Vitro Techniques Methods to study reactions or processes taking place in an artificial environment outside the living organism. In Vitro Test,In Vitro Testing,In Vitro Tests,In Vitro as Topic,In Vitro,In Vitro Technique,In Vitro Testings,Technique, In Vitro,Techniques, In Vitro,Test, In Vitro,Testing, In Vitro,Testings, In Vitro,Tests, In Vitro,Vitro Testing, In

Related Publications

S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
November 2006, Cancer genetics and cytogenetics,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
September 1997, Cancer genetics and cytogenetics,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
August 1989, Cancer genetics and cytogenetics,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
October 1979, Nature,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
May 1986, Annals of the rheumatic diseases,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
November 1980, The New England journal of medicine,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
June 1991, Cancer genetics and cytogenetics,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
March 1992, Research in veterinary science,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
August 1983, Cancer genetics and cytogenetics,
S Knees-Matzen, and M Roser, and U Reimers, and U Ehlert, and M Weichenthal, and E W Breitbart, and H W Rüdiger
January 1977, Annual review of genetics,
Copied contents to your clipboard!