BIOMAT Database Logo BIOMAT Database Logo

Firefly luciferase inhibition. 2010

João M M Leitão, and Joaquim C G Esteves da Silva
Centro de Investigação em Química (CIQ-UP), Grupo de Ciências Biológicas e Bioanalíticas, Faculdade de Farmácia da Universidade de Coimbra, Polo das Ciências da Saúde, 3000-548 Coimbra, Portugal.

Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of an electronically excited species (oxyluciferin), inorganic pyrophosphate (PPi), carbon dioxide and adenosine monophosphate (AMP). Luc also catalyzes other non-luminescent reactions, which can interfere with the light production mechanism. Following electronic relaxation, the excited oxyluciferin emits radiation in the visible region of the electromagnetic spectrum (550-570 nm). Among the various possible compounds, several classes of inhibitory substances interfere with the activity of this enzyme: here, we consider substrate-related compounds, intermediates or products of the Luc catalyzed reactions, in addition to anesthetics and, fatty acids. This review summarizes the main inhibitors of Luc and the corresponding inhibition kinetic parameters.

UI MeSH Term Description Entries
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D011719 Pyrazines A heterocyclic aromatic organic compound with the chemical formula C4H4N2. Pyrazine
D002384 Catalysis The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction. Catalyses
D004791 Enzyme Inhibitors Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction. Enzyme Inhibitor,Inhibitor, Enzyme,Inhibitors, Enzyme
D005227 Fatty Acids Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed) Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D000473 Alkanes The generic name for the group of aliphatic hydrocarbons Cn-H2n+2. They are denoted by the suffix -ane. (Grant & Hackh's Chemical Dictionary, 5th ed) Alkane
D000777 Anesthetics Agents capable of inducing a total or partial loss of sensation, especially tactile sensation and pain. They may act to induce general ANESTHESIA, in which an unconscious state is achieved, or may act locally to induce numbness or lack of sensation at a targeted site. Anesthetic,Anesthetic Agents,Anesthetic Drugs,Anesthetic Effect,Anesthetic Effects,Agents, Anesthetic,Drugs, Anesthetic,Effect, Anesthetic,Effects, Anesthetic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D048888 Fireflies The family Lampyidae, which are bioluminescent BEETLES. They contain FIREFLY LUCIFERIN and LUCIFERASES. Oxidation of firefly luciferin results in luminescence. Lampyridae,Firefly,Luciola,Photinus,Luciolas
D049409 Luciferases, Firefly Luciferases from FIREFLIES, usually Photinus, that oxidizes FIREFLY LUCIFERIN to cause emission of PHOTONS. Firefly Luciferase,Firefly Luciferases,Luciferase, Firefly

Related Publications

João M M Leitão, and Joaquim C G Esteves da Silva
Anion inhibition of firefly luciferase.
December 1970, Archives of biochemistry and biophysics,
João M M Leitão, and Joaquim C G Esteves da Silva
Firefly luciferase inhibition: a widely neglected problem.
January 2015, Archives of toxicology,
João M M Leitão, and Joaquim C G Esteves da Silva
Inhibition of firefly luciferase by alkane analogues.
January 2005, Biochimica et biophysica acta,
João M M Leitão, and Joaquim C G Esteves da Silva
Firefly luciferase.
January 1976, Advances in enzymology and related areas of molecular biology,
João M M Leitão, and Joaquim C G Esteves da Silva
Kinetics of product inhibition during firefly luciferase luminescence.
February 1977, Biochemistry,
João M M Leitão, and Joaquim C G Esteves da Silva
Crystalline firefly luciferase.
April 1956, Biochimica et biophysica acta,
João M M Leitão, and Joaquim C G Esteves da Silva
Cloning firefly luciferase.
January 1986, Methods in enzymology,
João M M Leitão, and Joaquim C G Esteves da Silva
Resveratrol inhibits firefly luciferase.
December 2006, Biochemical and biophysical research communications,
João M M Leitão, and Joaquim C G Esteves da Silva
[Inhibition of luciferase from the firefly Luciola mingrelica by ATP analogs].
August 1982, Biokhimiia (Moscow, Russia),
João M M Leitão, and Joaquim C G Esteves da Silva
Kinetics of inhibition of firefly luciferase by oxyluciferin and dehydroluciferyl-adenylate.
September 2008, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology,
Copied contents to your clipboard!