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We analyzed the expression of class-I antigens in ex vivo human tumor cells by isoelectric focusing (IEF) the anti-class-I mAb W6/32 immunoprecipitates prepared from cell lysates. Out of 42 experiments, 27 were technically successful. The patient's blood lymphocytes were used as controls. In vitro exposure of the tumor cells to IFN gamma and TNF alpha elevated class-I antigen expression. In 11 cases, defects in MHC-class-I-antigen expression were observed. In 2 cases the antigens were detected only in the cytokine-treated tumor samples, probably due to a defect in the association between beta 2m and class-I heavy chains. Selective changes in the expression of alleles were seen in 10 cases and might involve HLA A, B and C antigens. Alterations in class-I expression as compared with the lymphocytes were observed in 9 of 13 cases in which the tumor cells were collected from metastases, and only in 2 of 14 primary tumors.
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
February 1991,
Tissue antigens,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
October 1989,
Pathology,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
January 1990,
Immunogenetics,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
October 2002,
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
June 1991,
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
April 1989,
Journal of immunology (Baltimore, Md. : 1950),
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
January 1995,
Clinical & experimental metastasis,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
February 1987,
Tissue antigens,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
February 1989,
Transplantation proceedings,
P Wang, and
F Vánky, and
S L Li, and
Z Végh, and
U Persson, and
E Klein
April 2005,
Cancer immunology, immunotherapy : CII,
