Biomaterial Database

Mass spectrometry imaging of small molecules using matrix-enhanced surface-assisted laser desorption/ionization mass spectrometry (ME-SALDI-MS). 2010

Qiang Liu, and Yongsheng Xiao, and Lin He

Department of Pathology & Lab Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI substrates exhibit reduced background in the low-mass region that is often crowded in conventional matrix-assisted laser desorption/ionization (MALDI) due to matrix fragmentation; a benefit renders SALDI-MS attractive in imaging low-mass species. Practical application of SALDI-MS, however, is hindered by its unsatisfied detection sensitivity for most compounds. With aims of improving MS imaging resolution and sensitivity of low-mass species, we describe an experimental protocol using a hybrid ionization method, termed matrix-enhanced SALDI (ME-SALDI), to detect crucial low-mass species with their spatial distribution in mouse brain tissue.

UI	MeSH Term	Description	Entries
D008853	Microscopy	The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.	Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002531	Cerebellum	The part of brain that lies behind the BRAIN STEM in the posterior base of skull (CRANIAL FOSSA, POSTERIOR). It is also known as the "little brain" with convolutions similar to those of CEREBRAL CORTEX, inner white matter, and deep cerebellar nuclei. Its function is to coordinate voluntary movements, maintain balance, and learn motor skills.	Cerebella,Corpus Cerebelli,Parencephalon,Cerebellums,Parencephalons
D003952	Diagnostic Imaging	Any visual display of structural or functional patterns of organs or tissues for diagnostic evaluation. It includes measuring physiologic and metabolic responses to physical and chemical stimuli, as well as ultramicroscopy.	Imaging, Diagnostic,Imaging, Medical,Medical Imaging
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013058	Mass Spectrometry	An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.	Mass Spectroscopy,Spectrometry, Mass,Spectroscopy, Mass,Spectrum Analysis, Mass,Analysis, Mass Spectrum,Mass Spectrum Analysis,Analyses, Mass Spectrum,Mass Spectrum Analyses,Spectrum Analyses, Mass
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D054022	Cerebrum	Derived from TELENCEPHALON, cerebrum is composed of a right and a left hemisphere. Each contains an outer cerebral cortex and a subcortical basal ganglia. The cerebrum includes all parts within the skull except the MEDULLA OBLONGATA, the PONS, and the CEREBELLUM. Cerebral functions include sensorimotor, emotional, and intellectual activities.	Cerebra,Cerebral Hemispheres,Left Cerebral Hemisphere,Right Cerebral Hemisphere,Cerebral Hemisphere,Cerebral Hemisphere, Left,Cerebral Hemisphere, Right