Isolation and molecular identification of Cronobacter spp. from powdered infant formula (PIF) in Bangladesh. 2010

Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh.

Cronobacter spp. formerly known as Enterobacter sakazakii is an occasional contaminant of powdered infant formula (PIF). This pathogen has been associated with out-breaks of a rare form of infant meningitis, necrotizing enterocolitis (NEC), bacteremia and neonate deaths. The organism is ranked by the International Commission for Microbiological Specifications for Foods (ICMSF) as a 'Severe hazard for restricted populations, life threatening or substantial chronic sequelae or long duration'. Present study aimed to isolate Cronobacter spp. from PIF and clinical samples, such as blood, stool and CSF collected from 93 neonates and child patients, age ranged from 0 to 24months. We did not detect Cronobacter spp. in any of these samples. Later 32 PIF samples collected from retail markets in Bangladesh were tested for the presence of Cronobacter spp. Of these only one was found to be contaminated with Cronobacter sp. This is the first case of Cronobacter contaminated PIF found in Bangladesh to be reported. The organism was successfully identified based on its typical culture characteristics, producing blue-green colonies on chromogenic DFI agar and also by a standardized conventional PCR assay targeting the alpha glucosidase and 16S rRNA gene sequence of Cronobacter sp. The 16S rRNA gene was partially sequenced to provide for the phylogenetic analysis of this isolate (DA01) and found to cluster with some other Cronobacter isolates in the phylogram.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D007225 Infant Food Food processed and manufactured for the nutritional health of children in their first year of life. Food, Infant,Foods, Infant,Infant Foods
D007231 Infant, Newborn An infant during the first 28 days after birth. Neonate,Newborns,Infants, Newborn,Neonates,Newborn,Newborn Infant,Newborn Infants
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010802 Phylogeny The relationships of groups of organisms as reflected by their genetic makeup. Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D003257 Consumer Product Safety The sum total of measures taken and regulatory policies enacted to ensure the safe use of consumer products. Safety, Consumer Product,Product Approval,Product Approvals,Product Safety, Consumer
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D005506 Food Contamination The presence in food of harmful, unpalatable, or otherwise objectionable foreign substances, e.g. chemicals, microorganisms or diluents, before, during, or after processing or storage. Food Adulteration,Adulteration, Food,Adulterations, Food,Contamination, Food,Contaminations, Food,Food Adulterations,Food Contaminations
D005516 Food Microbiology The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept. Microbiology, Food
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
March 2012, Journal of food protection,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
June 2014, Foodborne pathogens and disease,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
March 2023, Foods (Basel, Switzerland),
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
June 2010, International journal of food microbiology,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
January 2022, Frontiers in microbiology,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
December 2009, International journal of food microbiology,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
January 2016, Acta medica Iranica,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
January 2016, Food science and biotechnology,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
December 2021, Journal of AOAC International,
Ashfaqul Hoque, and Tahmeed Ahmed, and Mohammad Shahidullah, and Anowar Hossain, and Abdul Mannan, and Khaled Noor, and Kamrun Nahar, and Mohammad Ilias, and Dilruba Ahmed
May 2017, Journal of the science of food and agriculture,
Copied contents to your clipboard!