A sensitive and selective liquid chromatography/tandem mass spectrometry method for determination of MLN8237 in human plasma. 2010

Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
Division of Clinical Pharmacology and Therapeutics, The Children's Hospital of Philadelphia, USA.

We describe a selective and a highly sensitive high-performance liquid chromatography-electron spray ionization-collision induced dissociation-tandem mass spectrometry (HPLC-ESI-CID-MS/MS) assay for the Aurora A kinase inhibitor MLN8237 in human plasma. The intra-day precision based on the standard deviation of replicates of quality control samples ranged from 0.2 to 4% and with accuracy ranging from 96 to 102%. The inter-day precision ranged from 0.5 to 7% and the accuracy ranged from 93 to 105%. Stability studies showed that MLN8237 was stable both during the expected conditions for sample preparation and storage. The lower limit of quantification for MLN8237 was 5 ng/mL. The analytical method showed excellent sensitivity, precision, and accuracy. This method is robust and is being successfully employed in a Children's Oncology Group Phase 1 Consortium study of MLN8237 in children with cancer.

UI MeSH Term Description Entries
D011743 Pyrimidines A family of 6-membered heterocyclic compounds occurring in nature in a wide variety of forms. They include several nucleic acid constituents (CYTOSINE; THYMINE; and URACIL) and form the basic structure of the barbiturates.
D002853 Chromatography, Liquid Chromatographic techniques in which the mobile phase is a liquid. Liquid Chromatography
D004355 Drug Stability The chemical and physical integrity of a pharmaceutical product. Drug Shelf Life,Drugs Shelf Lives,Shelf Life, Drugs,Drug Stabilities,Drugs Shelf Life,Drugs Shelf Live,Life, Drugs Shelf,Shelf Life, Drug,Shelf Live, Drugs,Shelf Lives, Drugs
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001381 Azepines Seven membered heterocyclic rings containing a NITROGEN atom. Hexamethyleneimines
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D016018 Least-Squares Analysis A principle of estimation in which the estimates of a set of parameters in a statistical model are those quantities minimizing the sum of squared differences between the observed values of a dependent variable and the values predicted by the model. Rietveld Refinement,Analysis, Least-Squares,Least Squares,Analyses, Least-Squares,Analysis, Least Squares,Least Squares Analysis,Least-Squares Analyses,Refinement, Rietveld
D017346 Protein Serine-Threonine Kinases A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors. Protein-Serine-Threonine Kinases,Serine-Threonine Protein Kinase,Serine-Threonine Protein Kinases,Protein-Serine Kinase,Protein-Serine-Threonine Kinase,Protein-Threonine Kinase,Serine Kinase,Serine-Threonine Kinase,Serine-Threonine Kinases,Threonine Kinase,Kinase, Protein-Serine,Kinase, Protein-Serine-Threonine,Kinase, Protein-Threonine,Kinase, Serine-Threonine,Kinases, Protein Serine-Threonine,Kinases, Protein-Serine-Threonine,Kinases, Serine-Threonine,Protein Kinase, Serine-Threonine,Protein Kinases, Serine-Threonine,Protein Serine Kinase,Protein Serine Threonine Kinase,Protein Serine Threonine Kinases,Protein Threonine Kinase,Serine Threonine Kinase,Serine Threonine Kinases,Serine Threonine Protein Kinase,Serine Threonine Protein Kinases
D047428 Protein Kinase Inhibitors Agents that inhibit PROTEIN KINASES. Protein Kinase Inhibitor,Inhibitor, Protein Kinase,Inhibitors, Protein Kinase,Kinase Inhibitor, Protein,Kinase Inhibitors, Protein

Related Publications

Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
December 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
April 2004, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
May 2003, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
March 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
January 2003, Rapid communications in mass spectrometry : RCM,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
February 2005, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
June 2010, Acta pharmaceutica (Zagreb, Croatia),
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
October 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
January 2013, PloS one,
Emily Lipsitz, and Ganesh Moorthy, and Yael Mosse, and Elizabeth Fox, and Peter C Adamson
June 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Copied contents to your clipboard!