Ultrastructural morphology of equine adipose-derived mesenchymal stem cells. 2010

Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
Department of Biopathological Science and Animal and Food Hygiene, University of Perugia, Perugia, Italy. luisa.pascucci@unipg.it

Mesenchymal stem cells are a virtually ubiquitous population of adult stem cells, able to differentiate into various tissue lineages. As they are multipotent and easy to grow in culture, they are at present considered very attractive candidates for tissue repair and gene therapy. With the exception of a few reports, mesenchymal stem cell morphology has been widely disregarded in the past years. In this paper we discuss the establishment of mesenchymal stem cell cultures from equine adipose tissue and describe their fine structure by transmission electron microscopy. The cultured cells revealed a fibroblastoid appearance and were characterized by an eccentric nucleus with multiple nucleoli, dense cytoplasm rich in ribosomes, a rough endoplasmic reticulum with dilated cisternae, elongated mitochondria and heterogeneous vacuolar inclusions. In addition, they were often interconnected by adhesion structures located on the cell body and on cytoplasmic processes contacting other cells. The features observed are evocative of an undifferentiated cellular phenotype and of an intense synthetic and metabolic activity.

UI MeSH Term Description Entries
D010012 Osteogenesis The process of bone formation. Histogenesis of bone including ossification. Bone Formation,Ossification, Physiologic,Endochondral Ossification,Ossification,Ossification, Physiological,Osteoclastogenesis,Physiologic Ossification,Endochondral Ossifications,Ossification, Endochondral,Ossifications,Ossifications, Endochondral,Osteoclastogeneses,Physiological Ossification
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D006736 Horses Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest. Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D000273 Adipose Tissue Specialized connective tissue composed of fat cells (ADIPOCYTES). It is the site of stored FATS, usually in the form of TRIGLYCERIDES. In mammals, there are two types of adipose tissue, the WHITE FAT and the BROWN FAT. Their relative distributions vary in different species with most adipose tissue being white. Fatty Tissue,Body Fat,Fat Pad,Fat Pads,Pad, Fat,Pads, Fat,Tissue, Adipose,Tissue, Fatty
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015415 Biomarkers Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, ENVIRONMENTAL EXPOSURE and its effects, disease diagnosis; METABOLIC PROCESSES; SUBSTANCE ABUSE; PREGNANCY; cell line development; EPIDEMIOLOGIC STUDIES; etc. Biochemical Markers,Biological Markers,Biomarker,Clinical Markers,Immunologic Markers,Laboratory Markers,Markers, Biochemical,Markers, Biological,Markers, Clinical,Markers, Immunologic,Markers, Laboratory,Markers, Serum,Markers, Surrogate,Markers, Viral,Serum Markers,Surrogate Markers,Viral Markers,Biochemical Marker,Biologic Marker,Biologic Markers,Clinical Marker,Immune Marker,Immune Markers,Immunologic Marker,Laboratory Marker,Marker, Biochemical,Marker, Biological,Marker, Clinical,Marker, Immunologic,Marker, Laboratory,Marker, Serum,Marker, Surrogate,Serum Marker,Surrogate End Point,Surrogate End Points,Surrogate Endpoint,Surrogate Endpoints,Surrogate Marker,Viral Marker,Biological Marker,End Point, Surrogate,End Points, Surrogate,Endpoint, Surrogate,Endpoints, Surrogate,Marker, Biologic,Marker, Immune,Marker, Viral,Markers, Biologic,Markers, Immune
D046529 Microscopy, Electron, Transmission Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen. Electron Diffraction Microscopy,Electron Microscopy, Transmission,Microscopy, Electron Diffraction,Transmission Electron Microscopy,Diffraction Microscopy, Electron,Microscopy, Transmission Electron
D048430 Cell Shape The quality of surface form or outline of CELLS. Cell Shapes,Shape, Cell,Shapes, Cell

Related Publications

Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
April 2018, Research in veterinary science,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
August 2011, Veterinary research communications,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
January 2019, Bratislavske lekarske listy,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
December 2021, Cells,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
December 2015, Cell medicine,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
December 2009, Veterinary immunology and immunopathology,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
January 2022, Stem cell research & therapy,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
December 2014, Research in veterinary science,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
December 2011, Veterinary research communications,
Luisa Pascucci, and Francesca Mercati, and Carla Marini, and Piero Ceccarelli, and Cecilia Dall'Aglio, and Vera Pedini, and Anna M Gargiulo
August 2012, BMC veterinary research,
Copied contents to your clipboard!