Biomaterial Database

Purification, characterization and toxicity profile of ricin isoforms from castor beans. 2010

Payal Sehgal, and M Khan, and O Kumar, and R Vijayaraghavan

Division of Pharmacology and Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior, India.

The castor seed contains the toxin ricin, one of the most poisonous naturally occurring toxins. The whole of the plant is poisonous, however the seeds are considered the major source of ricin. Ricin exists in different forms in beans of different origin. We investigated the presence of ricin in different isoforms and elucidate some of their structural and biological features isolated from the castor seeds. The isoforms were sub fractionated into ricin I, II and III by chromatography. Their molecular weights lie between 60-65 kDa with difference in their relative electrophoretic mobility. An acidic native PAGE of ricin isoforms at pH 2.9 was performed. Ricin I, II and III are highly cytotoxic against Vero cell line with IC(50) values of 60, 30 and 8 ng/ml respectively. Difference in cytotoxicity of isoforms was confirmed through hemagglutination assay, ricin III caused high degree of hemolysis. The preliminary in vivo toxicity studies showed that ricin III is highly toxic. Immunological studies revealed that anti-ricin I and II antibodies are cross reactive with all the ricin variants, whereas the anti-ricin III antibody is highly specific. The present study shows that anti-ricin I and II antibodies can be used for detection of entire ricin isoforms.

UI	MeSH Term	Description	Entries
D007928	Lethal Dose 50	The dose amount of poisonous or toxic substance or dose of ionizing radiation required to kill 50% of the tested population.	LD50,Dose 50, Lethal
D008297	Male		Males
D010936	Plant Extracts	Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.	Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D010947	Plants, Toxic	Plants or plant parts which are harmful to man or other animals.	Plants, Poisonous,Plant, Poisonous,Plant, Toxic,Poisonous Plant,Poisonous Plants,Toxic Plant,Toxic Plants
D002367	Ricinus communis	A species in the family EUPHORBIACEAE that is the source of CASTOR OIL.	Castor Bean,Castor Bean Plant,Castor Oil Plant,Ricinus sanguineus,Bean, Castor,Beans, Castor,Castor Bean Plants,Castor Beans,Castor Oil Plants,Plant, Castor Bean,Plant, Castor Oil,Plants, Castor Bean,Plants, Castor Oil
D002470	Cell Survival	The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.	Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002522	Chlorocebus aethiops	A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.	African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D002619	Chemical Warfare Agents	Chemicals that are used to cause the disturbance, disease, or death of humans during WARFARE.	Agents, Chemical Warfare,Warfare Agents, Chemical,Agent, Chemical Warfare,Chemical Warfare Agent,Warfare Agent, Chemical
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D005591	Chemical Fractionation	Separation of a mixture in successive stages, each stage removing from the mixture some proportion of one of the substances, for example by differential solubility in water-solvent mixtures. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Fractionation, Chemical,Chemical Fractionations,Fractionations, Chemical