BIOMAT Database Logo BIOMAT Database Logo

A new, rapid, one-step method for the isolation of platelets from human blood. 1990

T C Ford, and J Graham, and D Rickwood
Department of Cellular and Molecular Sciences, St. Georges Hospital Medical School, London, UK.

We describe a new, rapid method for the isolation of platelets from human blood using a single centrifugation step through Nycodenz, an inert, nontoxic, nonionic medium. As judged by aggregation and nucleotide release studies, the platelets are recovered in high yield and in excellent functional condition.

UI MeSH Term Description Entries
D010974 Platelet Aggregation The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS. Aggregation, Platelet
D001792 Blood Platelets Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation. Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002469 Cell Separation Techniques for separating distinct populations of cells. Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002499 Centrifugation, Density Gradient Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000244 Adenosine Diphosphate Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position. ADP,Adenosine Pyrophosphate,Magnesium ADP,MgADP,Adenosine 5'-Pyrophosphate,5'-Pyrophosphate, Adenosine,ADP, Magnesium,Adenosine 5' Pyrophosphate,Diphosphate, Adenosine,Pyrophosphate, Adenosine
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2

Related Publications

T C Ford, and J Graham, and D Rickwood
A rapid one-step method for the isolation of human granulocytes from whole blood.
May 1982, Scandinavian journal of clinical and laboratory investigation,
T C Ford, and J Graham, and D Rickwood
Flow cytometric analysis of CD41-labeled platelets isolated by the rapid, one-step OptiPrep method from human blood.
May 2005, Cytometry. Part A : the journal of the International Society for Analytical Cytology,
T C Ford, and J Graham, and D Rickwood
A new one-step method for the isolation of human mononuclear cells.
December 1990, Journal of immunological methods,
T C Ford, and J Graham, and D Rickwood
One-step method for endothelial cell isolation from large human blood vessels using fibrin glue.
June 2000, Laboratory investigation; a journal of technical methods and pathology,
T C Ford, and J Graham, and D Rickwood
A rapid centrifugation method for the isolation of polymorphonuclear leucocytes from human blood.
January 1981, Acta physiologica Academiae Scientiarum Hungaricae,
T C Ford, and J Graham, and D Rickwood
A rapid one-step extraction procedure for the isolation of ubiquitin from human erythrocytes for antibody production.
January 1991, Preparative biochemistry,
T C Ford, and J Graham, and D Rickwood
A rapid and simple one step method for isolation of poly(A)+ RNA from cells in monolayer.
October 1990, Endocrinology,
T C Ford, and J Graham, and D Rickwood
A one-step method for counting leukocytes and platelets.
October 1966, Technical bulletin of the Registry of Medical Technologists. American Society of Clinical Pathologists. Registry of Medical Technologists,
T C Ford, and J Graham, and D Rickwood
Development of a rapid, one-step screening method for the isolation of presumptive proteolytic enterococci.
January 2017, Journal of microbiological methods,
T C Ford, and J Graham, and D Rickwood
[Rapid method of leukocyte isolation from the whole human blood].
October 1974, Rinsho byori. The Japanese journal of clinical pathology,
Copied contents to your clipboard!