BIOMAT Database Logo BIOMAT Database Logo

In vitro testing for anti-inflammatory properties of compounds employing peripheral blood mononuclear cells freshly isolated from healthy donors. 2011

M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Division of Biological Chemistry, Biocenter, Innsbruck Medical University, Fritz Pregl Strasse 3, 6020 Innsbruck, Austria.

BACKGROUND Inflammation is crucially involved in a variety of diseases like autoimmune syndromes, cardiovascular and neurodegenerative disorders, cancer, sepsis and allograft rejection. METHODS Freshly isolated human peripheral blood mononuclear cells (PBMCs) are used as a screening assay for anti-inflammatory properties of compounds. Determinations of neopterin production by ELISA and of tryptophan degradation by HPLC are used as read-outs. Results are compared with further markers of immune response and oxidative stress. RESULTS Phytohaemagglutinin induced significant tryptophan degradation and neopterin formation in PBMC, which correlated with IFN-γ, TNF-α, soluble cytokine receptors and isoprostane-8. Addition of vitamin C and E suppressed the responses dose-dependently. CONCLUSIONS The determination of tryptophan degradation and neopterin production in PBMC reflects various pro- and anti-inflammatory cascades that are of relevance also in patients. It constitutes a robust and reliable approach to screen anti-inflammatory or immunosuppressive drugs and may improve throughput, speed and cost-effectiveness in drug discovery.

UI MeSH Term Description Entries
D007249 Inflammation A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function. Innate Inflammatory Response,Inflammations,Inflammatory Response, Innate,Innate Inflammatory Responses
D007371 Interferon-gamma The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES. Interferon Type II,Interferon, Immune,gamma-Interferon,Interferon, gamma,Type II Interferon,Immune Interferon,Interferon, Type II
D007963 Leukocytes, Mononuclear Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules. Mononuclear Leukocyte,Mononuclear Leukocytes,PBMC Peripheral Blood Mononuclear Cells,Peripheral Blood Human Mononuclear Cells,Peripheral Blood Mononuclear Cell,Peripheral Blood Mononuclear Cells,Leukocyte, Mononuclear
D008934 Mitogens Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed) Mitogen,Phytomitogen,Phytomitogens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000893 Anti-Inflammatory Agents Substances that reduce or suppress INFLAMMATION. Anti-Inflammatory Agent,Antiinflammatory Agent,Agents, Anti-Inflammatory,Agents, Antiinflammatory,Anti-Inflammatories,Antiinflammatories,Antiinflammatory Agents,Agent, Anti-Inflammatory,Agent, Antiinflammatory,Agents, Anti Inflammatory,Anti Inflammatories,Anti Inflammatory Agent,Anti Inflammatory Agents
D014364 Tryptophan An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals. Ardeydorm,Ardeytropin,L-Tryptophan,L-Tryptophan-ratiopharm,Levotryptophan,Lyphan,Naturruhe,Optimax,PMS-Tryptophan,Trofan,Tryptacin,Tryptan,Tryptophan Metabolism Alterations,ratio-Tryptophan,L Tryptophan,L Tryptophan ratiopharm,PMS Tryptophan,ratio Tryptophan
D014409 Tumor Necrosis Factor-alpha Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS. Cachectin,TNF-alpha,Tumor Necrosis Factor Ligand Superfamily Member 2,Cachectin-Tumor Necrosis Factor,TNF Superfamily, Member 2,TNFalpha,Tumor Necrosis Factor,Cachectin Tumor Necrosis Factor,Tumor Necrosis Factor alpha
D047889 Receptors, Tumor Necrosis Factor, Type II A tumor necrosis factor receptor subtype that is expressed primarily in IMMUNE SYSTEM cells. It has specificity for membrane-bound form of TUMOR NECROSIS FACTORS and mediates intracellular-signaling through TNF RECEPTOR ASSOCIATED FACTORS. Antigens, CD120b,CD120b Antigens,Receptors, Tumor Necrosis Factor, Member 1B,Tumor Necrosis Factor Receptor Superfamily, Member 1B,Tumor Necrosis Factor Receptor Type II,CD 120b Antigen,CD120b Antigen,TNF-R2,TNF-R75,TNF-RII,TNF-sR75,TNFR p75,TNFR p80,TNFR2,TNFRSF1B Receptor,Tumor Necrosis Factor Receptor 2,Tumor Necrosis Factor Receptor 75,Tumor Necrosis Factor Receptor Type 2,120b Antigen, CD,Antigen, CD 120b,Antigen, CD120b,Receptor, TNFRSF1B
D053645 Interleukin-2 Receptor alpha Subunit A low affinity interleukin-2 receptor subunit that combines with the INTERLEUKIN-2 RECEPTOR BETA SUBUNIT and the INTERLEUKIN RECEPTOR COMMON GAMMA-CHAIN to form a high affinity receptor for INTERLEUKIN-2. Antigens, CD25,CD25 Antigens,Interleukin-2 Receptors alpha,Tac P55 Peptide,CD25 Antigen,IL-2Ralpha,Interleukin-2 Receptor alpha,Interleukin-2 Receptor alpha Chain,Interleukin-2Ralpha,alpha-subunit, Receptor, Interleukin-2,Antigen, CD25,IL 2Ralpha,Interleukin 2 Receptor alpha,Interleukin 2 Receptor alpha Chain,Interleukin 2 Receptor alpha Subunit,Interleukin 2 Receptors alpha,Interleukin 2Ralpha,Receptor alpha, Interleukin-2,Receptors alpha, Interleukin-2

Related Publications

M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.
June 2005, BMC musculoskeletal disorders,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors.
June 2023, Stem cell research,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Assessment of chicken peripheral blood mononuclear cells isolated from freshly drawn blood versus 24 h refrigerated blood.
September 2021, Veterinary world,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Sumatriptan increases the proliferation of peripheral blood mononuclear cells from HIV-infected individuals and healthy blood donors in vitro.
January 2000, APMIS : acta pathologica, microbiologica, et immunologica Scandinavica,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
[Pro-inflammatory and anti-inflammatory cytokine responses of peripheral blood mononuclear cells in apparently healthy subjects].
November 2014, Nan fang yi ke da xue xue bao = Journal of Southern Medical University,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Normal value for the direct micronucleus test on peripheral blood mononuclear cells from healthy donors.
December 2002, Analytical and quantitative cytology and histology,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
[Cytotoxic properties of mononuclear cells from the peripheral blood of healthy persons].
January 1985, Eksperimentalna meditsina i morfologiia,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
The effect of disodium cromoglycate on in vitro proliferation of peripheral blood mononuclear cells from allergic and healthy donors.
November 1992, Scandinavian journal of immunology,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Prevalence of viable Chlamydia pneumoniae in peripheral blood mononuclear cells of healthy blood donors.
July 2004, Transfusion,
M Jenny, and M Klieber, and D Zaknun, and S Schroecksnadel, and K Kurz, and M Ledochowski, and H Schennach, and Dietmar Fuchs
Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples.
January 2023, Genes,
Copied contents to your clipboard!