-transparent.png){kind=logo}
Cells lacking the enzyme thymidine kinase (LMTK- cells) have been transformed to a kinase-positive phenotype using sheared herpes simplex virus (HSV) DNA, and the enzyme found in these transformed cells is HSV-specific. One of the cell lines is able to complement the functional defect found in two temperature-sensitive mutants of HSV 1, and reversion of the cells to a thymidine kinase-negative phenotype results in the loss of this capability. The HSV thymidine kinase gene can also be introduced into LMTK- cells using DNA extracted from transformed cells, and the high efficiency of this procedure suggests that the state of the virus DNA in transformed cells is different from that of DNA in virus particles.
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
January 1980,
Journal of virology,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
January 1978,
IARC scientific publications,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
November 1980,
Somatic cell genetics,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
June 1982,
Molecular and cellular biology,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
April 1975,
Somatic cell genetics,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
November 1974,
Journal of virology,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
January 1978,
IARC scientific publications,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
March 1990,
Cytotechnology,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
January 1984,
The Journal of general virology,
A C Minson, and
P Wildy, and
A Buchan, and
G Darby
January 1978,
IARC scientific publications,
