-transparent.png){kind=logo}
The expression of large (pre-S1), middle (pre-S2), major S (S) polypeptides of the envelope (HBs) and X peptides of hepatitis B virus (HBV) was investigated in 37 liver specimens with chronic hepatitis B by indirect immunoperoxidase staining. Primary antisera utilized were polyclonal ones against HBs (poly-HBs), core (HBc) and X and monoclonal ones against pre-S1, pre-S2 and S with (particle-S) or without (peptide-S) conformational structure. The localization of HBs proteins in hepatocytes was classified into three types: diffuse, membranous and inclusion. The peptide-S and pre-S2 were expressed at nearly the same frequency as poly-HBs in all types, whereas particle-S was found less frequently (18/29 cases) in the inclusion type, and pre-S1 was recognized relatively rarely (9/33 cases) in the membranous type. As for staining intensity, peptide-S and pre-S2 were almost identical to poly-HBs which stained the most strongly among all three staining types. Particle-S was similar to poly-HBs in the membranous type, but was weak in the inclusion type in the majority. While pre-S1 was stained in a similar intensity to poly-HBs in the diffuse and inclusion types, it was weak or negative in the membranous type. Thus, envelope particles indicated by particle-S staining appeared to be located most frequently in the membranous type, but their assembly might be suppressed in the inclusion type where pre-S1 was well expressed. The X peptide was more frequently detected in the liver with serum HBe antigen and/or HBV DNA. The X peptide was stained exclusively in the cytoplasm of hepatocytes and was correlated with the cytoplasmic HBc antigen. The X peptide was not observed differently between cases with and those without cirrhosis. This suggests that the expression of X peptide tends to occur with virus replication but not with disease progression.
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
November 1992,
Gut,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
January 1996,
The National medical journal of India,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
September 1990,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
October 1990,
Journal of medical virology,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
April 1989,
Zhonghua nei ke za zhi,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
February 2015,
Medical microbiology and immunology,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
January 1990,
Hepatology (Baltimore, Md.),
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
November 1988,
Virology,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
February 1988,
Journal of medical virology,
K Suzuki, and
T Uchida, and
T Shikata, and
M Moriyama, and
Y Arakawa, and
M Mizokami, and
F Mima
December 2009,
Antimicrobial agents and chemotherapy,
