One-step multiplex reverse-transcriptase PCR for detecting pandemic (H1N1) 2009 influenza virus. 2011

Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
Virology Division, National Veterinary Research and Quarantine Service, Ministry for Food, Agriculture, Forestry and Fisheries, Gyeonggi-do, Republic of Korea. shinyk@nvrqs.go.kr

Pandemic (H1N1) 2009 influenza has spread throughout the world since April 2009 and has caused many human deaths since its first report in humans. Pandemic (H1N1) 2009 influenza virus was first identified in a Canadian pig herd in April 2009 and has been reported in more than ten countries, including Korea. We developed a one-step multiplex reverse transcriptase polymerase chain reaction (RT-PCR) assay based on the matrix gene that discriminates pandemic (H1N1) 2009 influenza virus from endemic swine influenza viruses. The sensitivity of this assay was 100 copies of in vitro-transcribed target RNA and 0.01 tissue culture infective dose (TCID(50)/ml) of virus and was as high as those of conventional influenza A virus common matrix reverse transcriptase PCR assays and real-time reverse transcriptase PCR assays (1 to 200 copies) developed for detecting pandemic (H1N1) 2009 influenza viruses from human and pig samples. This one-step multiplex RT-PCR assay would be a good tool in monitoring pandemic (H1N1) 2009 influenza virus among pig herds on a regular basis.

UI MeSH Term Description Entries
D007251 Influenza, Human An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia. Grippe,Human Flu,Human Influenza,Influenza in Humans,Influenza,Flu, Human,Human Influenzas,Influenza in Human,Influenzas,Influenzas, Human
D009976 Orthomyxoviridae Infections Virus diseases caused by the ORTHOMYXOVIRIDAE. Orthomyxovirus Infections,Infections, Orthomyxoviridae,Infections, Orthomyxovirus,Swine Influenza,Infection, Orthomyxoviridae,Infection, Orthomyxovirus,Influenza, Swine,Orthomyxoviridae Infection,Orthomyxovirus Infection
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D013047 Specific Pathogen-Free Organisms Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free. Pathogen-Free Organisms,Specific Pathogen Free,Organism, Pathogen-Free,Organism, Specific Pathogen-Free,Organisms, Pathogen-Free,Organisms, Specific Pathogen-Free,Pathogen Free Organisms,Pathogen Free, Specific,Pathogen Frees, Specific,Pathogen-Free Organism,Pathogen-Free Organism, Specific,Pathogen-Free Organisms, Specific,Specific Pathogen Free Organisms,Specific Pathogen-Free Organism
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog

Related Publications

Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
September 2011, Journal of clinical microbiology,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
January 2011, Journal of virological methods,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
April 2011, Journal of clinical microbiology,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
May 2010, Journal of clinical microbiology,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
December 2009, Journal of clinical microbiology,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
March 2010, Journal of virological methods,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
December 2010, Journal of virological methods,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
July 2020, Analytical biochemistry,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
July 2011, Journal of clinical microbiology,
Yeun-Kyung Shin, and Soon-Seek Yoon, and Jae-Young Song, and Joon-Bae Kim, and Do Thi Thu Hang, and Jin-Woo Park, and Min-Young Kim, and Seong-Hee Kim, and Choi-Kyu Park, and O-Soo Li
September 2010, Journal of virological methods,
Copied contents to your clipboard!