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Succinylcholine is frequently employed in surgical procedures as a pre-anaesthetic. However, the lack of existing metabolic activity for this compound in some individuals entails a substantial risk. Normally, the risk is assessed indirectly through the measurement of pseudocholinesterase activity using other substrates, such as acetylcholine, rather than the agent itself. Thus, a method was devised to assess directly the hydrolysis of succinylcholine in human plasma samples. This method was applied to plasma samples derived from healthy men, healthy women, pregnant women and patients with silent pseudocholinesterasaemia.
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