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MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait.
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
January 2001,
In vitro cellular & developmental biology. Animal,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
September 2015,
Biotechnology letters,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
March 1990,
Cell biology international reports,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
May 1986,
Cell biology international reports,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
April 1987,
Carcinogenesis,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
March 2006,
Biotechnology letters,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
June 1998,
Mutation research,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
November 1994,
Gene,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
April 2015,
Biotechnology letters,
Kathryn C Johnson, and
Nitya M Jacob, and
Peter Morin Nissom, and
Matthias Hackl, and
Lim Hseuh Lee, and
Miranda Yap, and
Wei-Shou Hu
January 2013,
PloS one,
