Restoration of spermatogenesis after transplantation of c-Kit positive testicular cells in the fowl. 2010

Pavel Trefil, and Murray R Bakst, and Haifeng Yan, and Jiří Hejnar, and Jiří Kalina, and Jitka Mucksová
BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, a.s. 254 49 Jílové u Prahy, Czech Republic. trefil@bri.cz

Transplantation of male germ line cells into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis. We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit-positive spermatogonia as donor cells. Dispersed c-Kit positive testicular cells from 16 to 17 week-old pubertal donors were transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient's testes with c-Kit positive donor testicular cells, which resulted in the production of functional heterologous spermatozoa. Using manual semen collection, the first sperm production in the recipient males was observed about nine weeks after the transplantation. The full reproduction cycle was accomplished by artificial insemination of hens and hatching of chickens.

UI MeSH Term Description Entries
D007315 Insemination, Artificial Artificial introduction of SEMEN or SPERMATOZOA into the VAGINA to facilitate FERTILIZATION. Artificial Insemination,Eutelegenesis,Artificial Inseminations,Eutelegeneses,Inseminations, Artificial
D008297 Male Males
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D005260 Female Females
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D013093 Spermatogonia Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES. Spermatophores,Spermatogonias,Spermatophore
D013245 Sterilization, Reproductive Procedures to block or remove all or part of the genital tract for the purpose of rendering individuals sterile, incapable of reproduction. Surgical sterilization procedures are the most commonly used. There are also sterilization procedures involving chemical or physical means. Reproductive Sterilization,Voluntary Sterilization,Female Sterilization,Female Sterilization, Voluntary,Male Sterilization,Male Sterilization, Voluntary,Female Sterilizations,Female Sterilizations, Voluntary,Male Sterilizations,Male Sterilizations, Voluntary,Reproductive Sterilizations,Sterilization, Female,Sterilization, Male,Sterilization, Voluntary,Sterilizations, Female,Sterilizations, Male,Sterilizations, Reproductive,Sterilizations, Voluntary,Voluntary Female Sterilization,Voluntary Female Sterilizations,Voluntary Male Sterilization,Voluntary Male Sterilizations,Voluntary Sterilizations
D013737 Testis The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS. Testicles,Testes,Testicle
D017690 Cell Transplantation Transference of cells within an individual, between individuals of the same species, or between individuals of different species. Transplantation, Cell

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