Oct1 regulates trophoblast development during early mouse embryogenesis. 2010

Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
Max Planck Institute for Molecular Biomedicine, Department of Cell and Developmental Biology, Röntgenstrasse, 20 48149 Münster, Germany.

Oct1 (Pou2f1) is a transcription factor of the POU-homeodomain family that is unique in being ubiquitously expressed in both embryonic and adult mouse tissues. Although its expression profile suggests a crucial role in multiple regions of the developing organism, the only essential function demonstrated so far has been the regulation of cellular response to oxidative and metabolic stress. Here, we describe a loss-of-function mouse model for Oct1 that causes early embryonic lethality, with Oct1-null embryos failing to develop beyond the early streak stage. Molecular and morphological analyses of Oct1 mutant embryos revealed a failure in the establishment of a normal maternal-embryonic interface due to reduced extra-embryonic ectoderm formation and lack of the ectoplacental cone. Oct1(-/-) blastocysts display proper segregation of trophectoderm and inner cell mass lineages. However, Oct1 loss is not compatible with trophoblast stem cell derivation. Importantly, the early gastrulation defect caused by Oct1 disruption can be rescued in a tetraploid complementation assay. Oct1 is therefore primarily required for the maintenance and differentiation of the trophoblast stem cell compartment during early post-implantation development. We present evidence that Cdx2, which is expressed at high levels in trophoblast stem cells, is a direct transcriptional target of Oct1. Our data also suggest that Oct1 is required in the embryo proper from late gastrulation stages onwards.

UI MeSH Term Description Entries
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D011247 Pregnancy The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH. Gestation,Pregnancies
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004622 Embryo, Mammalian The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS. Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260 Female Females
D005865 Gestational Age The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination. Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D014327 Trophoblasts Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA). Cytotrophoblasts,Syncytiotrophoblasts,Trophoblast,Cytotrophoblast,Syncytiotrophoblast

Related Publications

Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
February 1978, Journal of embryology and experimental morphology,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
December 2015, Molecular endocrinology (Baltimore, Md.),
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
January 2016, Developmental cell,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
January 2020, Reproduction (Cambridge, England),
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
November 2006, Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
January 1999, Developmental genetics,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
April 2003, Current biology : CB,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
August 2008, Current opinion in genetics & development,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
January 2008, Molecular and cellular endocrinology,
Vittorio Sebastiano, and Mathieu Dalvai, and Luca Gentile, and Karin Schubart, and Julien Sutter, and Guang-Ming Wu, and Natalia Tapia, and Daniel Esch, and Jin-Young Ju, and Karin Hübner, and Marcos Jesus Arauzo Bravo, and Hans Robert Schöler, and Fatima Cavaleri, and Patrick Matthias
January 1983, Haematology and blood transfusion,
Copied contents to your clipboard!