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In 20 fecal specimens stored at ambient temperature (30-40 degrees C) for 3-7 days, substantial reductions in hookworm ova load were observed when a sensitive egg counting method (modified Kato's technique) was employed. Aliquots to which sodium azide (3 mg/g of fecal matter) was added showed considerably less reduction. A larger study on 120 specimens confirmed that there was no reduction up to 3 days. Significant decreases, however, occurred by 5 days, and these could not be prevented even by doubling the sodium azide dose (to 6 mg). It is recommended that in epidemiological studies of hookworm infestation in tropical countries, 3 mg of sodium azide should be added per g of fecal matter within 6 h of collection and the specimen tested within 3 days.
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
August 1979,
Clinical chemistry,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
June 1991,
Annals of tropical medicine and parasitology,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
September 2017,
International journal of legal medicine,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
December 1945,
The Puerto Rico journal of public health and tropical medicine,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
January 1975,
Clinical chemistry,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
June 1985,
Parasitology,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
January 2007,
Water science and technology : a journal of the International Association on Water Pollution Research,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
December 1948,
The Journal of experimental zoology,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
January 1992,
Revista da Sociedade Brasileira de Medicina Tropical,
V Srinivasan, and
R Ramakrishnan, and
L Prabhakar
March 1948,
Journal of the American Medical Women's Association,
