PCR-linked reverse DNA hybridization using oligonucleotide-specific probes of rpoB for identification of Mycobacterium avium and Mycobacterium intracellulare. 2010

Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine, Seoul 120-752, South Korea.

A PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes (Avp and Intp) of Mycobacterium avium and Mycobacterium intracellulare, respectively, were evaluated for the differentiation and identification of M. avium and M. intracellulare culture isolates. Among the 504 culture isolates tested by this method, 48 strains showed positive results for M. avium and 60 strains showed positive results for M. intracellulare. The other 396 culture isolates showed negative results for both M. avium and M. intracellulare. These results were consistent with those obtained from partial rpoB (306 bp) sequence analysis and biochemical tests. The negative strains obtained by this DNA hybridization method were identified as M. tuberculosis (366 strains), M. peregrinum (11 strains), M. abscessus (9 strains), M. fortuitum (8 strains), and M. flavescens (2 strains) by rpoB DNA sequence analysis. Due to the high sensitive and specific result obtained by this assay, we suggest that this PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes of M. avium and M. intracellulare would be used for the rapid and precise method for differentiation and identification of M. avium and M. intracellulare.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009162 Mycobacterium avium A bacterium causing tuberculosis in domestic fowl and other birds. In pigs, it may cause localized and sometimes disseminated disease. The organism occurs occasionally in sheep and cattle. It should be distinguished from the M. avium complex, which infects primarily humans.
D009164 Mycobacterium Infections Infections with bacteria of the genus MYCOBACTERIUM. Infections, Mycobacterium,Infection, Mycobacterium,Mycobacterium Infection
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012321 DNA-Directed RNA Polymerases Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992). DNA-Dependent RNA Polymerases,RNA Polymerases,Transcriptases,DNA-Directed RNA Polymerase,RNA Polymerase,Transcriptase,DNA Dependent RNA Polymerases,DNA Directed RNA Polymerase,DNA Directed RNA Polymerases,Polymerase, DNA-Directed RNA,Polymerase, RNA,Polymerases, DNA-Dependent RNA,Polymerases, DNA-Directed RNA,Polymerases, RNA,RNA Polymerase, DNA-Directed,RNA Polymerases, DNA-Dependent,RNA Polymerases, DNA-Directed
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D015269 Mycobacterium avium Complex A complex that includes several strains of M. avium. M. intracellulare is not easily distinguished from M. avium and therefore is included in the complex. These organisms are most frequently found in pulmonary secretions from persons with a tuberculous-like mycobacteriosis. Strains of this complex have also been associated with childhood lymphadenitis and AIDS; M. avium alone causes tuberculosis in a variety of birds and other animals, including pigs. Battey Bacillus,MAIC,Mycobacterium avium-intracellulare,Mycobacterium avium-intracellulare Complex,Mycobacterium intracellulare,Nocardia intracellularis

Related Publications

Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
October 2004, Journal of microbiological methods,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
August 1990, Journal of clinical microbiology,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
May 1989, Journal of clinical microbiology,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
May 1993, Kekkaku : [Tuberculosis],
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
November 1996, Journal of clinical microbiology,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
February 1993, Kekkaku : [Tuberculosis],
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
October 1987, Diagnostic microbiology and infectious disease,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
July 1989, Journal of clinical microbiology,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
November 1991, Journal of clinical microbiology,
Sun-Joon Bai, and Jin-Seong Eum, and Young-Doo Park, and Sang-Ho Chung, and Yoon-Hoh Kook, and Seong-Karp Hong
May 1996, Journal of clinical microbiology,
Copied contents to your clipboard!