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An enzyme-linked immunosorbent assay (ELISA) for detecting Staphylococcus aureus antibody in bovine milk samples was examined for repeatability. A set of 51 bovine milk samples from 4 universities with confirmed culture results was assembled, and a panel of 30 milk samples was randomly selected. When the selected panel was tested at the collection laboratory, there was 97% agreement between the ELISA and the culture test. The panel was tested with the ELISA by the 4 university laboratories. Results were scored by both visual and optical density reader methods. When compared to reference ELISA results, the university laboratory ELISA results showed an agreement of 99.8% for negative samples, 98% for positive samples, and 99% for all samples. Additional studies on 19 milk samples that cultured positive for bacteria other than S. aureus showed 100% specificity. Overall comparison of ELISA and culture results showed high agreement between the 2 techniques. Disagreement appeared to result from explainable differences in antibody and bacterial levels and not from errors in either of the 2 techniques.
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American journal of veterinary research,
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D S Adams
March 1984,
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J S McDonald, and
P M Sears, and
D S Adams
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T Matsushita, and
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Journal of clinical microbiology,
T Matsushita, and
R P Dinsmore, and
R J Eberhart, and
G M Jones, and
J S McDonald, and
P M Sears, and
D S Adams
September 2013,
New Zealand veterinary journal,
