-transparent.png){kind=logo}
A polypeptide growth factor has been partially purified from medium conditioned by the human adrenocortical carcinoma cell line SW13. This factor, designated h-TGFe, stimulates anchorage-independent growth of the SW13 cells. Similar activity was observed in human milk, and in conditioned media from seven of 14 epithelial cell lines. The SW13-derived activity is stable to low pH and 8M urea but labile to dithiothreitol and 2% sodium dodecyl sulfate. Human TGFe does not bind to heparin and fails to stimulate growth of endothelial cells in monolayer culture. The apparent molecular weight of h-TGFe is 59k by size exclusion chromatography in the presence of 8M urea and the activity binds strongly to cation exchangers. The activity elutes at 15-30% acetonitrile from a C18 reverse-phase column and has been partially purified by using a four-step chromatographic procedure. TGFe appears to be a novel growth factor produced by many epithelial cells and tissues.
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
August 1989,
Clinica chimica acta; international journal of clinical chemistry,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
November 1985,
Biochemical and biophysical research communications,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
January 1989,
Archivum immunologiae et therapiae experimentalis,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
January 1986,
Lymphokine research,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
September 1987,
Cancer research,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
February 1981,
Analytical biochemistry,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
February 1986,
Cancer research,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
August 1979,
Proceedings of the National Academy of Sciences of the United States of America,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
August 1986,
Endocrinologia japonica,
D J Dunnington, and
R G Scott, and
M A Anzano, and
R Greig
November 1986,
Biochemical and biophysical research communications,
