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Serum samples of 62 African patients who had clinical manifestations of HIV-1 infection but were seronegative for HIV-1 by ELISA (Organon) were subsequently further tested by another HIV-1 ELISA test (Wellcozyme), HIV-1 IgG Western blot, HIV-1 antigen detection and HIV-2 ELISA. Patients' lymphocytes were cultured for HIV-1 and 2. Because of limited quantities of serum available all tests were not performed on all samples. Seven (26%) of 27 sera of patients meeting the WHO clinical case definition of AIDS were Western-blot-positive. In contrast, of 35 patients' sera with possible HIV related disease, only one (3%) was Western blot positive (P = 0.02) and none of 75 sera from HIV-1 ELISA (Organon) seronegative blood donors (P less than 0.01) were Western blot positive. Of 30 HIV-1 ELISA (Organon) seronegative patients tested with the HIV-1 ELISA Wellcozyme assay only one was seropositive (this patient's serum was also Western blot positive). Of 17 HIV-1 ELISA (Organon) seronegative patients tested, HIV-1 antigen was found in 1 case (6%) (this patient's serum was Western blot negative). None of the 34 patients tested by HIV-2 serology was HIV-2 seropositive. HIV-1 was isolated by culture in 3 (21%) of 14 HIV-1 ELISA seronegative patients (sera of the 3 patients were Western blot negative). In total, 12 (19%) of 62 HIV-1 ELISA (Organon) seronegative patients were found to be positive for HIV, either by Western blot HIV antigen testing or viral culture.(ABSTRACT TRUNCATED AT 250 WORDS)
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
May 1993,
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I Lebughe, and
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T C Quinn, and
W L Heyward, and
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March 1989,
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May 1991,
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P Piot
June 1988,
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R Colebunders, and
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T C Quinn, and
W L Heyward, and
P Piot
January 1992,
Journal of acquired immune deficiency syndromes,
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
April 1991,
AIDS (London, England),
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
December 1987,
Annales de la Societe belge de medecine tropicale,
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
January 1999,
AIDS (London, England),
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
December 1990,
AIDS (London, England),
R Colebunders, and
H Francis, and
M M Duma, and
G van der Groen, and
I Lebughe, and
B Kapita, and
T C Quinn, and
W L Heyward, and
P Piot
September 1990,
AIDS (London, England),
