Biomaterial Database

Physicochemical properties of the cytoplasmic triiodothyronine binding protein from tadpole liver and tail fin. 1978

R C Jaffe

The sedimentation velocity, gel filtration properties and pattern of elution from ion exchange gels of the cytoplasmic triiodothyronine (T3) binding protein from Rana catesbeiana liver and tail fin cytosol were determined. The T3 binding protein in liver cytosol had a sedimentation coefficient of 4.4S on sucrose gradients and a Stokes radius from Sephadex gel filtration of 38.2 +/- 4.2 A. From these two values a molecular weight of 71,700 +/- 4,100 and a frictional ratio of 1.28 +/- 0.05 for the T3 binding protein from tadpole liver has been calculated. The liver T3 binding protein was eluted from DEAE--Sephadex gels at a salt concentration of 0.233 +/- 0.017 M NaCl. The sedimentation coefficient, Stokes radius and salt requirement for elution from DEAE--Sephadex of the T3 binding protein from tail fin cytosol were essentially identical (4.4S, 35.2 +/- 4.2 A, nd 0.213 +/- 0.015 M NaCl respectively. The calculated molecular weight is 66,100 +/- 7,900 and the frictional ratio is 1.21 +/- 0.10 for for the tail fin T3 binding protein. The great similarity in the physicochemical properties of the T3 binding protein from the liver and tail fin implies that the T3 binding protein in each tissue is similar if not identical. The possible reason for the differences in the dissociation constants previously reported for the binding of T3 in the two tissues is discussed.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011892	Rana catesbeiana	A species of the family Ranidae (true frogs). The only anuran properly referred to by the common name "bullfrog", it is the largest native anuran in North America.	Bullfrog,Bullfrogs,Rana catesbeianas,catesbeiana, Rana
D011956	Receptors, Cell Surface	Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.	Cell Surface Receptor,Cell Surface Receptors,Hormone Receptors, Cell Surface,Receptors, Endogenous Substances,Cell Surface Hormone Receptors,Endogenous Substances Receptors,Receptor, Cell Surface,Surface Receptor, Cell
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002845	Chromatography	Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.	Chromatographies
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001001	Anura	An order of the class Amphibia, which includes several families of frogs and toads. They are characterized by well developed hind limbs adapted for jumping, fused head and trunk and webbed toes. The term "toad" is ambiguous and is properly applied only to the family Bufonidae.	Bombina,Frogs and Toads,Salientia,Toad, Fire-Bellied,Toads and Frogs,Anuras,Fire-Bellied Toad,Fire-Bellied Toads,Salientias,Toad, Fire Bellied,Toads, Fire-Bellied
D013623	Tail	An extension of the posterior of an animal body beyond the TORSO.	Tails
D014284	Triiodothyronine	A T3 thyroid hormone normally synthesized and secreted by the thyroid gland in much smaller quantities than thyroxine (T4). Most T3 is derived from peripheral monodeiodination of T4 at the 5' position of the outer ring of the iodothyronine nucleus. The hormone finally delivered and used by the tissues is mainly T3.	Liothyronine,T3 Thyroid Hormone,3,3',5-Triiodothyronine,Cytomel,Liothyronine Sodium,Thyroid Hormone, T3