-transparent.png){kind=logo}
The present study was undertaken to assess the presence of tumor necrosis factor (TNF)-alpha mRNA and protein in circulating human blood monocytes and to study the TNF-alpha gene expression in human monocytes isolated by continuous Percoll gradient fractionation. The technique of RNA isolation directly from the blood samples was used to study TNF-alpha mRNA expression in circulating human blood leukocytes. It was shown that human blood leukocytes of healthy donors contained no presynthesized pool of TNF-alpha mRNA as well as no TNF-alpha protein. It was found that early pretreatment with cycloheximide interferes with TNF-alpha mRNA induction by Staphylococcus aureus.
Related Publications
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
January 1989,
Journal of biological regulators and homeostatic agents,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
November 1992,
The Journal of clinical investigation,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
August 1999,
Molecular cell biology research communications : MCBRC,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
September 1991,
Biulleten' eksperimental'noi biologii i meditsiny,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
March 2005,
Journal of periodontology,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
March 1989,
Proceedings of the National Academy of Sciences of the United States of America,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
January 1991,
Life sciences,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
March 2002,
The Journal of trauma,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
May 1990,
Cancer research,
N I Misuno, and
O A Osipovich, and
A B Sudarikov, and
G L Idelson, and
T S Kolesnikova, and
A V Panyutich, and
N N Voitenok
January 1989,
Immunology letters,
