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Crystallization and preliminary X-ray diffraction analysis of red clover necrotic mosaic virus. 2010
Stanton L Martin, and
Richard H Guenther, and
Tim L Sit, and
Paul D Swartz, and
Flora Meilleur, and
Steven A Lommel, and
Robert B Rose
Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA.
Red clover necrotic mosaic virus (RCNMV) is a species that belongs to the Tombusviridae family of plant viruses with a T = 3 icosahedral capsid. RCNMV virions were purified and were crystallized for X-ray analysis using the hanging-drop vapor-diffusion method. Self-rotation functions and systematic absences identified the space group as I23, with two virions in the unit cell. The crystals diffracted to better than 4 Å resolution but were very radiation-sensitive, causing rapid decay of the high-resolution reflections. The data were processed to 6 Å in the analysis presented here.
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