BIOMAT Database Logo BIOMAT Database Logo

Cotinine levels in serum and bronchoalveolar lavage fluid. 2010

Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Department of Chest Diseases, Ankara University School of Medicine, Ankara, Turkey.

Cotinine is a major metabolite of nicotine. This study was planned to investigate the relationship between bronchoalveolar lavage (BAL) fluid cotinine levels and serum cotinine levels in smokers and nonsmokers with various pulmonary diseases and to investigate whether these levels are affected by passive smoking. Serum and BAL fluid cotinine levels were measured in 27 patients. BAL cotinine levels were measured using a sensitive ELISA kit produced to measure cotinine in saliva. Plates were read by microuant (BioTek, USA) micro plate reader. All patient serum cotinine levels were detectable except for one nonsmoker patient. However, BAL fluid cotinine levels were measurable in only 6 patients (two of them were nonsmokers). A significant positive correlation was seen between serum and BAL fluid cotinine levels (r = 0.726; p = 0.000). Serum cotinine levels were significantly higher in present smokers than non-smokers (21.0 +/- 16.01; 5.35 +/- 7.65; p = 0.004). However, there were no significant differences in BAL fluid cotinine levels between smokers and nonsmokers. Passive smoking can increase nicotine metabolites in serum and other body fluids, including BAL fluid. Since BAL fluid and serum cotinine levels were well correlated, there is no need to use invasive procedures, such as bronchoscopy and expensive, time consuming BAL fluid analyses. Serum cotinine levels can give a rough idea of smoking status. BAL fluid cotinine meaurements should be done for only scientific reasons.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D001992 Bronchoalveolar Lavage Fluid Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung. Alveolar Lavage Fluid,Bronchial Lavage Fluid,Lung Lavage Fluid,Bronchial Alveolar Lavage Fluid,Lavage Fluid, Bronchial,Lavage Fluid, Lung,Pulmonary Lavage Fluid,Alveolar Lavage Fluids,Bronchial Lavage Fluids,Bronchoalveolar Lavage Fluids,Lavage Fluid, Alveolar,Lavage Fluid, Bronchoalveolar,Lavage Fluid, Pulmonary,Lavage Fluids, Alveolar,Lavage Fluids, Bronchial,Lavage Fluids, Bronchoalveolar,Lavage Fluids, Lung,Lavage Fluids, Pulmonary,Lung Lavage Fluids,Pulmonary Lavage Fluids
D001999 Bronchoscopy Endoscopic examination, therapy or surgery of the bronchi. Bronchoscopic Surgical Procedures,Surgical Procedures, Bronchoscopic,Bronchoscopic Surgery,Surgery, Bronchoscopic,Bronchoscopic Surgeries,Bronchoscopic Surgical Procedure,Bronchoscopies,Surgeries, Bronchoscopic,Surgical Procedure, Bronchoscopic
D003367 Cotinine The N-glucuronide conjugate of cotinine is a major urinary metabolite of NICOTINE. It thus serves as a biomarker of exposure to tobacco SMOKING. It has CNS stimulating properties. Scotine
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012907 Smoking Willful or deliberate act of inhaling and exhaling SMOKE from burning substances or agents held by hand. Smoking Behaviors,Smoking Habit,Behavior, Smoking,Behaviors, Smoking,Habit, Smoking,Habits, Smoking,Smoking Behavior,Smoking Habits
D014028 Tobacco Smoke Pollution Contamination of the air by tobacco smoke. Air Pollution, Tobacco Smoke,Environmental Pollution, Tobacco Smoke,Passive Smoking,Smoking, Passive,Environmental Smoke Pollution, Tobacco,Environmental Tobacco Smoke Pollution,Involuntary Smoking,Second Hand Smoke,Secondhand Smoke,Secondhand Smoking,Hand Smoke, Second,Hand Smokes, Second,Involuntary Smokings,Passive Smokings,Pollution, Tobacco Smoke,Pollutions, Tobacco Smoke,Second Hand Smokes,Secondhand Smokes,Secondhand Smokings,Smoke Pollution, Tobacco,Smoke Pollutions, Tobacco,Smoke, Second Hand,Smoke, Secondhand,Smokes, Second Hand,Smokes, Secondhand,Smoking, Involuntary,Smoking, Secondhand,Smokings, Involuntary,Smokings, Passive,Smokings, Secondhand,Tobacco Smoke Pollutions

Related Publications

Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
[Serum and bronchoalveolar lavage fluid neopterin levels in patients with bronchial asthma].
January 2003, Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Serum and bronchoalveolar lavage fluid nitric oxide levels in diffuse systemic sclerosis.
March 2009, Rheumatology international,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Enzyme levels in bronchoalveolar lavage fluid and serum of active pulmonary tuberculosis patients.
January 1989, Enzyme,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Serum CA19-9 levels reflect bronchoalveolar lavage fluid neutrophil levels in idiopathic pulmonary fibrosis.
January 2001, Respiration; international review of thoracic diseases,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Levels of transferrin in bronchoalveolar lavage fluid in sarcoidosis.
April 2010, Lung,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Bronchoalveolar lavage fluid histamine levels in interstitial lung diseases.
December 1988, The American review of respiratory disease,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Immunoglobulin levels in bronchoalveolar lavage fluid from pigeon breeders.
July 1980, The Journal of laboratory and clinical medicine,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Bronchoalveolar lavage fluid and serum complement activity in pulmonary sarcoidosis.
August 1986, The American review of respiratory disease,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Interferon-gamma levels in serum and bronchoalveolar lavage fluid of mice infected with Bordetella pertussis.
March 1993, The Journal of infectious diseases,
Ozlem Erçen Diken, and Serap Unculu, and Demet Karnak, and Osman Cağlayan, and Julide Sedef Göçmen, and Oya Kayacan
Serum and bronchoalveolar lavage fluid levels of soluble B7H3 in patients with interstitial lung diseases.
June 2023, Respiratory medicine,
Copied contents to your clipboard!